ArticleAnalytical chemistry2020
Ensemble Sensing Using Single-Molecule DNA Copolymers.
Article in Analytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Modulating a Massive Set of Biomolecular Structures by Sono-Mechanical Force.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Stick-slip unfolding favors self-association of expanded HTT mRNA.Nature communications · 2024Article
- Dynamic Structures and Fast Transition Kinetics of Oxidized G-Quadruplexes.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Catalytic Relaxation of Kinetically Trapped Intermediates by DNA Chaperones.Journal of the American Chemical Society · 2024Article
- A mutation-sensitive, multiplexed and amplification-free detection of nucleic acids by stretching single-molecule tandem hairpin probes.Nucleic acids research · 2023Article
- Advances in ligase-based nucleic acid amplification technology for detecting gene mutations: a review.Molecular and cellular biochemistry · 2023Review
- Ensemble Force Spectroscopy of a G-Quadruplex Cluster on a Single-Molecule Platform.Biomacromolecules · 2022Article
- Short LNA-modified oligonucleotide probes as efficient disruptors of DNA G-quadruplexes.Nucleic acids research · 2022Article
- Single-Molecule Mechanochemical Sensing.Accounts of chemical research · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
While single-molecule sensing has offered ultimate mass sensitivity at the precision of individual molecules, it requires a longer time to detect analytes at lower concentrations when analyte binding to single-molecule probes becomes diffusion-limited. Here, we solved this accuracy problem in the concentration sensitivity determination by using single-molecule DNA homopolymers, in which up to 473 identical sensing elements (DNA hairpins) were introduced by rolling circle amplification. Surprisingly, the DNA homopolymers containing as few as 10 tandem hairpins displayed ensemble unfolding/refolding transitions, which were exploited to recognize microRNAs (miRNAs) that populated unfolded hairpins. Within 20 min, the femtomolar detection limit for miRNAs was observed, 6 orders of magnitude better than standalone hairpins. By incorporating different hairpin probes in an alternating DNA copolymer, multiplex recognition of different miRNAs was demonstrated. These DNA co-polymers represent new materials for innovative sensing strategies that combine the single-molecule precision with the accuracy of ensemble assays to determine concentration sensitivities.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.