ArticleJournal of applied genetics2020
Development of high-resolution melting PCR (HRM-PCR) assay to identify native fungal species associated with the wheat endosphere.
Article in Journal of applied genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- High-Resolution Melting Analysis for Differentiation ofMycobiology · 2025Article
- High-Resolution Melting assays development for discrimination of fungal pathogens causing Grapevine Trunk Diseases.PloS one · 2025Article
- Fungi Inhabiting the Wheat Endosphere.Pathogens (Basel, Switzerland) · 2021Review
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Authors and funding
4 authors.
Funding
Abstract
Understanding the complexity and biodiversity of fungal communities associated with the wheat endosphere can facilitate the identification of novel strains that might be beneficial to the host plant. However, the differentiation and taxonomic classification of the endosphere-associated fungi with respect to various cultivars and plant organs are challenging, time-consuming, and expensive, even with the use of molecular techniques. In the present work, we describe a fast, simple, and low-cost method based on high-resolution melting PCR (HRM-PCR) for the identification and differentiation of wheat endogenous fungal isolates. Using this approach, we differentiated 28 fungal isolates, which belonged to five different genera, namely Alternaria, Penicillium, Epicoccum, Fusarium, and Trichoderma. Furthermore, the results of the study revealed that this method can allow large-scale screening of cultured samples.
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