Evidence map›Paper›PMID 32828428›Full record

ArticleBioorganic & medicinal chemistry2020

Genetically encoding thyronine for fluorescent detection of peroxynitrite.

Shanshan Li, Bing Yang, Tomonori Kobayashi, Bingchen Yu, Jun Liu, Lei Wang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioorganic & medicinal chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. Review
  3. Update of the Pyrrolysyl-tRNA Synthetase/tRNAJournal of bacteriology · 2023
    Review
  4. Review
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Shanshan LiDepartment of Pharmaceutical Chemistry and the Cardiovascular Research Institute, University of California San Francisco, 555 Mission Bay Boulevard South, San Francisco, CA 94158, United States.
Bing YangDepartment of Pharmaceutical Chemistry and the Cardiovascular Research Institute, University of California San Francisco, 555 Mission Bay Boulevard South, San Francisco, CA 94158, United States.
Tomonori KobayashiDepartment of Pharmaceutical Chemistry and the Cardiovascular Research Institute, University of California San Francisco, 555 Mission Bay Boulevard South, San Francisco, CA 94158, United States.
Bingchen YuDepartment of Pharmaceutical Chemistry and the Cardiovascular Research Institute, University of California San Francisco, 555 Mission Bay Boulevard South, San Francisco, CA 94158, United States.
Jun LiuDepartment of Pharmaceutical Chemistry and the Cardiovascular Research Institute, University of California San Francisco, 555 Mission Bay Boulevard South, San Francisco, CA 94158, United States.
Lei WangDepartment of Pharmaceutical Chemistry and the Cardiovascular Research Institute, University of California San Francisco, 555 Mission Bay Boulevard South, San Francisco, CA 94158, United States. Electronic address: Lei.Wang2@ucsf.edu.
University of California, San Francisco · US

Funding

Adding New Covalent Bonds to Proteins in Live CellsR01GM118384 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Lei Wang · 2016 to 2026
$3.0M
NIGMS NIH HHS R01 GM118384
6 · The paper itself

Abstract

Peroxynitrite is a highly reactive oxidant effecting cell signaling and cell death. Here we report a fluorescent protein probe to selectively detect peroxynitrite. A novel unnatural amino acid, thyronine (Thy), was genetically encoded in E. coli and mammalian cells by evolving an orthogonal tRNA

Indexed as

Escherichia coliFluorescent DyesGreen Fluorescent ProteinsHEK293 CellsHeLa CellsHumansHydrogen PeroxideKineticsLimit of DetectionOxidation-ReductionPeroxynitrous AcidRNA, TransferThyroninesTyrosineFluorescent DyesGreen Fluorescent ProteinsHydrogen PeroxidePeroxynitrous AcidRNA, TransferThyroninesTyrosineFluorescenceGenetic code expansionPeroxynitriteThyronineUnnatural amino acid

Identifiers

PMID32828428
PMCPMC7461609
OpenAlexW3045686567

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.