ArticleCellular microbiology2020
Bacteroides fragilis fucosidases facilitate growth and invasion of Campylobacter jejuni in the presence of mucins.
Article in Cellular microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 28 citations in OpenAlex.
- Mucin-degrading gut bacteria: context-dependent roles in intestinal homeostasis and disease.Gut microbes · 2026Review
- Development of Fluorogenic Probes to Detect Glycan-Degrading Enzymes in Bacteria.ACS infectious diseases · 2026Article
- Article
- FucR Functions as a Transcriptional Regulator for L-Fucose Utilization inMicroorganisms · 2025Article
- Growth rates and metabolic traits differ by diarrhoeal manifestation inJournal of medical microbiology · 2025Article
- Emerging gut microbial glycoside hydrolase inhibitors.RSC chemical biology · 2025Review
- The relationship of N-glycosylation and porcine duodenal morphology and function.Journal of animal science · 2025Article
- Deciphering the gastrointestinal carriage ofInfection and immunity · 2024Review
- Activation of the l-fucose utilization cluster inHeliyon · 2024Article
- The role of the glycome in symbiotic host-microbe interactions.Glycobiology · 2023Review
- Activity-based protein profiling in microbes and the gut microbiome.Current opinion in chemical biology · 2023Review
- L-Fucose is involved in human-gut microbiome interactions.Applied microbiology and biotechnology · 2023Review
- Structure and function of microbial α-l-fucosidases: a mini review.Essays in biochemistry · 2023Review
- Chemoproteomic Approaches for Unraveling Prokaryotic Biology.Israel journal of chemistry · 2023Article
- Klebsiella pneumoniae l-Fucose Metabolism Promotes Gastrointestinal Colonization and Modulates Its Virulence Determinants.Infection and immunity · 2022Article
- Detection of Bacterial α-l-Fucosidases with anMolecules (Basel, Switzerland) · 2022Article
- Identification of α-L-fucosidase (ALFuc) of Blastocystis sp. subtypes ST1, ST2 and ST3.Revista do Instituto de Medicina Tropical de Sao Paulo · 2022Article
- Acute infection withFrontiers in cellular and infection microbiology · 2022Article
- Comparative Analysis of L-Fucose Utilization and Its Impact on Growth and Survival ofFrontiers in microbiology · 2022Article
- Mucin-Type O-Glycans: Barrier, Microbiota, and Immune Anchors in Inflammatory Bowel Disease.Journal of inflammation research · 2021Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The enteropathogenic bacterium, Campylobacter jejuni, was considered to be non-saccharolytic, but recently it emerged that l-fucose plays a central role in C. jejuni virulence. Half of C. jejuni clinical isolates possess an operon for l-fucose utilisation. In the intestinal tract, l-fucose is abundantly available in mucin O-linked glycan structures, but C. jejuni lacks a fucosidase enzyme essential to release the l-fucose. We set out to determine how C. jejuni can gain access to these intestinal l-fucosides. Growth of the fuc + C. jejuni strains, 129,108 and NCTC 11168, increased in the presence of l-fucose while fucose permease knockout strains did not benefit from additional l-fucose. With fucosidase assays and an activity-based probe, we confirmed that Bacteriodes fragilis, an abundant member of the intestinal microbiota, secretes active fucosidases. In the presence of mucins, C. jejuni was dependent on B. fragilis fucosidase activity for increased growth. Campylobacter jejuni invaded Caco-2 intestinal cells that express complex O-linked glycan structures that contain l-fucose. In infection experiments, C. jejuni was more invasive in the presence of B. fragilis and this increase is due to fucosidase activity. We conclude that C. jejuni fuc + strains are dependent on exogenous fucosidases for increased growth and invasion.
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