Evidence map›Paper›PMID 32827128›Full record

ArticleIn vitro cellular & developmental biology. Animal2020

Establishment and characterization of a new embryonic cell line from Helicoverpa armigera (Hübner).

Lin Zhou, Yunfei Li, Heyuan Wang, Yicheng Zhou, Zhihui Zhu

Abstract read
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In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Lin ZhouHubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Yunfei LiHubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Heyuan WangHubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Yicheng ZhouHubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Zhihui ZhuHubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China. zhihui@mail.hzau.edu.cn.ORCID http://orcid.org/0000-0002-6650-1241

Funding

National Natural Science Foundation of China No. 31872290 and 31572329
6 · The paper itself

Abstract

Here, a new cell line, Ha168, was established from Helicoverpa armigera eggs and has been stably subcultured for over 30 passages in TNM-FH medium supplemented with 10% fetal bovine serum. The cell line consists of round and spindle-shaped cells and several giant cells. The round cells, with a cell diameter of 14.30 ± 2.804 μm, account for 77% of the cells. DNA amplification fingerprinting, random amplified polymorphic DNA analysis, and analysis of the mitochondrial cytochrome c oxidase subunit I gene confirmed that the Ha168 cells were derived from H. armigera. Karyotype analysis revealed the average chromosome number of Ha168 cells to be 71. Growth curves at passage 25 were determined and demonstrated that the cell population doubling time is 56.8 h. No mycoplasma contamination was detected in the cell line. Ha168 cells can be infected by recombinant baculovirus AcMNPV-EGFP, and exogenous protein expression level in this cell line is 70% of that in the Sf9 cell line.

Indexed as

AnimalsBaculoviridaeCell Culture TechniquesCell LineCell ProliferationElectron Transport Complex IVEmbryo, NonmammalianGreen Fluorescent ProteinsKaryotypingMothsMycoplasmaRandom Amplified Polymorphic DNA TechniqueRecombinant ProteinsElectron Transport Complex IVenhanced green fluorescent proteinGreen Fluorescent ProteinsRecombinant ProteinsAcMNPV-EGFPCO I geneEmbryonic cell lineHelicoverpa armigeraMycoplasma

Identifiers

PMID32827128

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.