Evidence map›Paper›PMID 32826290›Full record

ArticleLife science alliance2020

Biochemical and functional characterization of a meiosis-specific Pch2/ORC AAA+ assembly.

María Ascensión Villar-Fernández, Richard Cardoso da Silva, Magdalena Firlej, Dongqing Pan, Elisabeth Weir, Annika Sarembe, Vivek B Raina, Tanja Bange, John R Weir, Gerben Vader

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Life science alliance, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

María Ascensión Villar-FernándezDepartment of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany.
Richard Cardoso da SilvaDepartment of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany.ORCID 0000-0003-2767-2053
Magdalena FirlejFriedrich Miescher Laboratory, Tübingen, Germany.
Dongqing PanDepartment of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany.ORCID 0000-0003-3701-8442
Elisabeth WeirDepartment of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany.
Annika SarembeDepartment of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany.
Vivek B RainaDepartment of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany.ORCID 0000-0003-0507-9208
Tanja BangeDepartment of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany.
John R WeirDepartment of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany.ORCID 0000-0002-6904-0284
Gerben VaderDepartment of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany gerben.vader@mpi-dortmund.mpg.de.ORCID 0000-0001-5729-0991
Max Planck Institute of Molecular Physiology · DEFriedrich Miescher Laboratory · DE

Funding

Wellcome Trust
6 · The paper itself

Abstract

Pch2 is a meiosis-specific AAA+ protein that controls several important chromosomal processes. We previously demonstrated that Orc1, a subunit of the ORC, functionally interacts with budding yeast Pch2. The ORC (Orc1-6) AAA+ complex loads the AAA+ MCM helicase to origins of replication, but whether and how ORC collaborates with Pch2 remains unclear. Here, we show that a Pch2 hexamer directly associates with ORC during the meiotic G2/prophase. Biochemical analysis suggests that Pch2 uses its non-enzymatic NH

Indexed as

Cell CycleCell Cycle ProteinsDNA HelicasesDNA ReplicationG2 Phase Cell Cycle CheckpointsMeiosisNuclear ProteinsOrigin Recognition ComplexProphaseProtein BindingReplication OriginSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSaccharomycetalesCell Cycle ProteinsDNA HelicasesNuclear ProteinsORC1 protein, S cerevisiaeOrigin Recognition ComplexPch2 protein, S cerevisiaeSaccharomyces cerevisiae Proteins

Identifiers

PMID32826290
PMCPMC7442955
OpenAlexW3080595898

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.