Evidence map›Paper›PMID 32824504›Full record

ArticleVeterinary sciences2020

Flow Cytometry-Detected Immunological Markers and on Farm Recorded Parameters in Composite Cow Milk as Related to Udder Health Status.

Giovanna De Matteis, Francesco Grandoni, Maria Carmela Scatà, Gennaro Catillo, Bianca Moioli, Luca Buttazzoni

Abstract read
In one paragraph

Article in Veterinary sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
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  4. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Giovanna De MatteisResearch Centre for Animal Production and Aquaculture, Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria (CREA), 00015 Monterotondo (Roma), Italy.ORCID 0000-0003-2963-3365
Francesco GrandoniResearch Centre for Animal Production and Aquaculture, Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria (CREA), 00015 Monterotondo (Roma), Italy.
Maria Carmela ScatàResearch Centre for Animal Production and Aquaculture, Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria (CREA), 00015 Monterotondo (Roma), Italy.ORCID 0000-0002-2626-7059
Gennaro CatilloResearch Centre for Animal Production and Aquaculture, Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria (CREA), 00015 Monterotondo (Roma), Italy.
Bianca MoioliResearch Centre for Animal Production and Aquaculture, Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria (CREA), 00015 Monterotondo (Roma), Italy.
Luca ButtazzoniResearch Centre for Animal Production and Aquaculture, Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria (CREA), 00015 Monterotondo (Roma), Italy.ORCID 0000-0002-0067-684X

Funding

Ministero delle Politiche Agricole Alimentari e Forestali D.M. 2763/7303/2015
6 · The paper itself

Abstract

Flow cytometry is a powerful technology used in many fields of cell biology. It is also used as a routine method to count somatic cells in milk and to characterize bovine milk leukocytes. In this study, we used flow cytometry to simultaneously assess the viability, the percentage of the single subsets of leukocytes and to quantify the expression of CD11b, an immunological marker of cell activation status. Immunological markers were then related with on farm recorded parameters as milk electrical conductivity (MEC) and average milk flow rate (MFR). Composite milk samples were collected from 43 cows, nine of which had naturally infected udders and 34 of which had no infected udders. First, the milk samples were classified according to bacteriological test in positive and negative. The results showed that the negative samples to bacteriological test had: (i) significantly higher percentages of live lymphocytes; (ii) significantly lower percentages of CD11b

Indexed as

cell viabilityelectrical conductivityflow cytometrymilk flow ratemilk somatic cells

Identifiers

PMID32824504
PMCPMC7559153

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.