ArticleInternational journal of molecular sciences2020
A Two-Step Immunomagnetic Microbead-Based Method for the Isolation of Human Primary Skin Telocytes/CD34+ Stromal Cells.
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 38 citations in OpenAlex.
- Telocytes in skeletal muscle: Emerging players in homeostasis and repair/regeneration.Histology and histopathology · 2026Review
- An Enriched Primary Culture Reveals Morphological Heterogeneity of Rat Testicular Telocytes.Cells · 2026Article
- Article
- Telocytes: history, origin, identification, structure, distribution, and functions.Histochemistry and cell biology · 2025Review
- Skin Telocyte Secretome as Conditioned Medium Prevents Profibrotic Differentiation of Skin Fibroblasts into Myofibroblasts.International journal of molecular sciences · 2025Article
- Developmental characteristics of cutaneous telocytes in late embryos of the silky fowl.European journal of histochemistry : EJH · 2024Article
- Review
- Article
- Telocytes: current methods of research, challenges and future perspectives.Cell and tissue research · 2024Review
- Vascular endothelial growth factor facilitates the effects of telocytes on tumor cell proliferation and migration.Frontiers in cell and developmental biology · 2024Article
- Telocytes/CD34+ Stromal Cells in the Normal, Hyperplastic, and Adenomatous Human Parathyroid Glands.International journal of molecular sciences · 2023Article
- Morphological and histochemical identification of telocytes in adult yak epididymis.Scientific reports · 2023Article
- Delimiting CD34+ Stromal Cells/Telocytes Are Resident Mesenchymal Cells That Participate in Neovessel Formation in Skin Kaposi Sarcoma.International journal of molecular sciences · 2023Article
- Review
- Comparison of the Behavior of Perivascular Cells (Pericytes and CD34+ Stromal Cell/Telocytes) in Sprouting and Intussusceptive Angiogenesis.International journal of molecular sciences · 2022Review
- Telocytes: An Emerging Component of Stem Cell Niche Microenvironment.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2021Review
- Scleroderma-like Impairment in the Network of Telocytes/CD34International journal of molecular sciences · 2021Article
- Cardiac Telocytes 16 Years on-What Have We Learned So Far, and How Close Are We to Routine Application of the Knowledge in Cardiovascular Regenerative Medicine?International journal of molecular sciences · 2021Review
- Telocytes promote hepatocellular carcinoma by activating the ERK signaling pathway and miR-942-3p/MMP9 axis.Cell death discovery · 2021Article
- Morphological Evidence of Telocytes in Skeletal Muscle Interstitium of Exercised and Sedentary Rodents.Biomedicines · 2021Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Telocytes (TCs), commonly referred to as TCs/CD34+ stromal cells, are a peculiar type of interstitial cells with distinctive morphologic traits that are supposed to exert several biological functions, including tissue homeostasis regulation, cell-to-cell signaling, immune surveillance, and reparative/regenerative effects. At present, the majority of studies investigating these cells are mainly descriptive and focus only on their morphology, with a consequent paucity of functional data. To gain relevant insight into the possible functions of TCs, in vitro analyses are clearly required, but currently, the protocols for TC isolation are only at the early stages and not fully standardized. In the present in vitro study, we describe a novel methodology for the purification of human primary skin TCs through a two-step immunomagnetic microbead-based cell separation (i.e., negative selection for CD31 followed by positive selection for CD34) capable of discriminating these cells from other connective tissue-resident cells on the basis of their different immunophenotypic features. Our experiments clearly demonstrated that the proposed method allows a selective purification of cells exhibiting the peculiar TC morphology. Isolated TCs displayed very long cytoplasmic extensions with a moniliform silhouette (telopodes) and presented an immunophenotypic profile (CD31-/CD34+/PDGFRα+/vimentin+) that unequivocally differentiates them from endothelial cells (CD31+/CD34+/PDGFRα-/vimentin+) and fibroblasts (CD31-/CD34-/PDGFRα+/vimentin+). This novel methodology for the isolation of TCs lays the groundwork for further research aimed at elucidating their functional properties and possible translational applications, especially in the field of regenerative medicine.
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