Evidence map›Paper›PMID 32824235›Full record

ArticleCancers2020

Exosomes-Mediated Transfer of Itga2 Promotes Migration and Invasion of Prostate Cancer Cells by Inducing Epithelial-Mesenchymal Transition.

Rofaida Gaballa, Hamdy E A Ali, Mohamed O Mahmoud, Johng S Rhim, Hamed I Ali, Heba F Salem, Mohammad Saleem, Mohamed A Kandeil, Stefan Ambs, Zakaria Y Abd Elmageed

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancers, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.

0numbers the graph read from it
0cells of the map it votes in
41citing papers in PubMed
5.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

41 citing papers in PubMed, 85 citations in OpenAlex.

  1. Review
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  9. The role and regulation of integrins in cell migration and invasion.Nature reviews. Molecular cell biology · 2025
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Rofaida GaballaDepartment of Pharmaceutical Sciences, Rangel College of Pharmacy, Texas A&M Health Science Center, College Station, TX 77843, USA.ORCID 0000-0003-1387-2118
Hamdy E A AliDepartment of Pharmaceutical Sciences, Rangel College of Pharmacy, Texas A&M Health Science Center, College Station, TX 77843, USA.
Mohamed O MahmoudDepartments of Biochemistry, Faculty of Pharmacy, Beni-Suef University; Beni-Suef 62521, Egypt.
Johng S RhimDepartment of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
Hamed I AliDepartment of Pharmaceutical Sciences, Rangel College of Pharmacy, Texas A&M Health Science Center, College Station, TX 77843, USA.
Heba F SalemDepartment of Pharmaceutics, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62521, Egypt.
Mohammad SaleemDepartment of Urology, Masonic Cancer Center, University of Minnesota, Minnesota, MN 55455, USA.ORCID 0000-0002-7422-7967
Mohamed A KandeilDepartment of Biochemistry, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62521, Egypt.
Stefan AmbsLaboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA.
Zakaria Y Abd ElmageedDepartment of Pharmaceutical Sciences, Rangel College of Pharmacy, Texas A&M Health Science Center, College Station, TX 77843, USA.ORCID 0000-0003-4031-0348
Beni-Suef University · EGTexas A&M Health Science Center · USNational Cancer Institute · USUniformed Services University of the Health Sciences · USUniversity of Minnesota Medical Center · US

Funding

The role of exRNA in Health Disparity of Prostate CancerR21CA194750 · NCI · TULANE UNIVERSITY OF LOUISIANA · PI ABD ELMAGEED, ZAKARIA · 2015 to 2016
$350k
NIH HHS R21CA194750
6 · The paper itself

Abstract

Although integrin alpha 2 subunit (ITGA2) mediates cancer progression and metastasis, its transfer by exosomes has not been investigated in prostate cancer (PCa). We aimed to determine the role of exosomal ITGA2 derived from castration-resistant PCa (CRPC) cells in promoting aggressive phenotypes in androgen receptor (AR)-positive cells. Exosomes were co-incubated with recipient cells and tested for different cellular assays. ITGA2 was enriched in exosomes derived from CRPC cells. Co-culture of AR-positive cells with CRPC-derived exosomes increased their proliferation, migration, and invasion by promoting epithelial-mesenchymal transition, which was reversed via ITGA2 knockdown or inhibition of exosomal uptake by methyl-β-cyclodextrin (MβCD). Ectopic expression of ITGA2 reproduced the effect of exosomal ITGA2 in PCa cells. ITGA2 transferred by exosomes exerted its effect within a shorter time compared to that triggered by its endogenous expression. The difference of ITGA2 protein expression in localized tumors and those with lymph node metastatic tissues was indistinguishable. Nevertheless, its abundance was higher in circulating exosomes collected from PCa patients when compared with normal subjects. Our findings indicate the possible role of the exosomal-ITGA2 transfer in altering the phenotype of AR-positive cells towards more aggressive phenotype. Thus, interfering with exosomal cargo transfer may inhibit the development of aggressive phenotype in PCa cells.

Indexed as

CRPCEMTexosomesIHCITGA2metastasisprostate cancer

Identifiers

PMID32824235
PMCPMC7466113
OpenAlexW3048986771

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.