Evidence map›Paper›PMID 32823670›Full record

ArticleInternational journal of molecular sciences2020

Suppression of

Zhihua Zhao, Hanshuo Zhang, Tuanlin Xiong, Junyi Wang, Di Yang, Dan Zhu, Juan Li, Ye Yang, Changhong Sun, Yuting Zhao and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Strategies for precise gene edits in mammalian cells.Molecular therapy. Nucleic acids · 2023
    Review
  5. Article
  6. Efficient gene editing through an intronic selection marker in cells.Cellular and molecular life sciences : CMLS · 2022
    Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Zhihua ZhaoState Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Molecular Medicine, Peking University, Beijing 100871, China.ORCID 0000-0001-7745-4615
Hanshuo ZhangDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China.
Tuanlin XiongCollege of Life Science, Peking University, Beijing 100871, China.
Junyi WangDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China.
Di YangDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China.
Dan ZhuDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China.
Juan LiDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China.
Ye YangDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China.
Changhong SunDepartment of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China.
Yuting ZhaoState Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Molecular Medicine, Peking University, Beijing 100871, China.
Jianzhong Jeff XiState Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Molecular Medicine, Peking University, Beijing 100871, China.
Peking University · CN

Funding

National Natural Science Foundation of China 81827809 to JX, 81421004 to JX
6 · The paper itself

Abstract

Homologous recombination (HR) is often used to achieve targeted gene integration because of its higher precision and operability compared with microhomology-mediated end-joining (MMEJ) or non-homologous end-joining (NHEJ). It appears to be inefficient for gene integration in animal cells and embryos due to occurring only during cell division. Here we developed genome-wide high-throughput screening and a subsequently paired crRNA library screening to search for genes suppressing homology-directed repair (HDR). We found that, in the reporter system, HDR cells with knockdown of

Indexed as

Gene Knock-In TechniquesRecombinational DNA RepairAnimalsBase SequenceCell LineEmbryo, MammalianGene EditingGenome, HumanHumansLuminescent ProteinsMembrane ProteinsMiceMicrofilament ProteinsRNAblue fluorescent protein, Aequorea victoriaLuminescent ProteinsMembrane ProteinsMicrofilament ProteinsRNAShrm protein, mouseShroom1 protein, humanCRISPR/Cas9gene editinghomologous recombinationknock-inSHROOM1

Identifiers

PMID32823670
PMCPMC7461567
OpenAlexW3048439210

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.