ArticleiScience2020
Adsorption Sequencing as a Rapid Method to Link Environmental Bacteriophages to Hosts.
Article in iScience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Hi-C Links Reveal Viral Activity and Infection Within the Free-Living Microbial Community of a Secondary Chlorophyll Maximum in the Eastern Tropical North Pacific.Environmental microbiology · 2026Article
- Tools and approaches to study the human gut virome: from the bench to bioinformatics.mSystems · 2026Review
- Cross-order detection of bacteriophage transduction in microbial communities using RNA barcoding.Nature communications · 2026Article
- Polyvalent phage GSP004 recognizes O-antigen polysaccharide receptors inJournal of virology · 2025Article
- The change of long tail fibers expanded the host range of a T5-like Salmonella phage and its application in milk.BMC microbiology · 2025Article
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- Biogeographic patterns and drivers of soil viromes.Nature ecology & evolution · 2024Article
- Bacteriophages of the OrderBiomolecules · 2023Review
- Review
- Mutualistic interplay between bacteriophages and bacteria in the human gut.Nature reviews. Microbiology · 2022Review
- Gut virome profiling identifies a widespread bacteriophage family associated with metabolic syndrome.Nature communications · 2022Article
- Thermal stress triggers productive viral infection of a key coral reef symbiont.The ISME journal · 2022Article
- Viral tag and grow: a scalable approach to capture and characterize infectious virus-host pairs.ISME communications · 2022Article
- Article
- Revisiting the rules of life for viruses of microorganisms.Nature reviews. Microbiology · 2021Review
- Examining horizontal gene transfer in microbial communities.Nature reviews. Microbiology · 2021Review
- Article
- Metaviromics coupled with phage-host identification to open the viral 'black box'.Journal of microbiology (Seoul, Korea) · 2021Review
- Sustainable Microbiome: a symphony orchestrated by synthetic phages.Microbial biotechnology · 2021Article
- Probing the "Dark Matter" of the Human Gut Phageome: Culture Assisted Metagenomics Enables Rapid Discovery and Host-Linking for Novel Bacteriophages.Frontiers in cellular and infection microbiology · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An important viromics challenge is associating bacteriophages to hosts. To address this, we developed adsorption sequencing (AdsorpSeq), a readily implementable method to measure phages that are preferentially adsorbed to specific host cell envelopes. AdsorpSeq thus captures the key initial infection cycle step. Phages are added to cell envelopes, adsorbed phages are isolated through gel electrophoresis, after which adsorbed phage DNA is sequenced and compared with the full virome. Here, we show that AdsorpSeq allows for separation of phages based on receptor-adsorbing capabilities. Next, we applied AdsorpSeq to identify phages in a wastewater virome that adsorb to cell envelopes of nine bacteria, including important pathogens. We detected 26 adsorbed phages including common and rare members of the virome, a minority being related to previously characterized phages. We conclude that AdsorpSeq is an effective new tool for rapid characterization of environmental phage adsorption, with a proof-of-principle application to Gram-negative host cell envelopes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.