ArticleStem cell reports2020
Canonical Wnt Pathway Controls mESC Self-Renewal Through Inhibition of Spontaneous Differentiation via β-Catenin/TCF/LEF Functions.
Article in Stem cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- Microarray and Single-Cell RNA Sequencing Reveals G-Protein Gene Expression Signatures of Spermatogonia Stem Cell.Stem cell reviews and reports · 2025Article
- Acetylation of lysine 49 on Ctnnb1 drives naïve pluripotency in murine stem cells by modulating Nanog function.PNAS nexus · 2025Article
- Advances in the Study of Pluripotent Stem Cells in Livestock.Cell proliferation · 2025Review
- Identification of core genes in acute kidney injury: evidence from multi-omics human transcriptomic data andTranslational andrology and urology · 2025Article
- β-catenin mediates endodermal commitment of human ES cells via distinct transactivation functions.Cell & bioscience · 2024Article
- The Wnt-dependent master regulator NKX1-2 controls mouse pre-implantation development.Stem cell reports · 2024Article
- LEF1 enhances β-catenin transactivation through IDR-dependent liquid-liquid phase separation.Life science alliance · 2023Article
- Endometrial stem/progenitor cells: Properties, origins, and functions.Genes & diseases · 2023Review
- The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency.Nature communications · 2023Article
- CHIR99021 Maintenance of the Cell Stemness by Regulating Cellular Iron Metabolism.Antioxidants (Basel, Switzerland) · 2023Article
- Highly efficient CRISPR-mediated large DNA docking and multiplexed prime editing using a single baculovirus.Nucleic acids research · 2022Article
- Cell-cell adhesions in embryonic stem cells regulate the stability and transcriptional activity of β-catenin.FEBS letters · 2022Article
- Advanced Molecular and Immunological Diagnostic Methods to Detect SARS-CoV-2 Infection.Microorganisms · 2022Review
- β-catenin perturbations control differentiation programs in mouse embryonic stem cells.iScience · 2022Article
- Wnt Signaling in the Breast: From Development to Disease.Frontiers in cell and developmental biology · 2022Review
- AXIN2 Reduces the Survival of Porcine Induced Pluripotent Stem Cells (piPSCs).International journal of molecular sciences · 2021Article
- DNMTs Play an Important Role in Maintaining the Pluripotency of Leukemia Inhibitory Factor-Dependent Embryonic Stem Cells.Stem cell reports · 2021Article
- MicroRNAs Modulate Signaling Pathways in Osteogenic Differentiation of Mesenchymal Stem Cells.International journal of molecular sciences · 2021Review
- Roles of Melatonin in Goat Hair Follicle Stem Cell Proliferation and Pluripotency Through Regulating the Wnt Signaling Pathway.Frontiers in cell and developmental biology · 2021Article
- Long Non-coding RNAs and MicroRNAs Interplay in Osteogenic Differentiation of Mesenchymal Stem Cells.Frontiers in cell and developmental biology · 2021Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 4 countries.
Funding
Abstract
The Wnt/β-catenin signaling pathway is a key regulator of embryonic stem cell (ESC) self-renewal and differentiation. Constitutive activation of this pathway has been shown to increase mouse ESC (mESC) self-renewal and pluripotency gene expression. In this study, we generated a novel β-catenin knockout model in mESCs to delete putatively functional N-terminally truncated isoforms observed in previous knockout models. We showed that aberrant N-terminally truncated isoforms are not functional in mESCs. In the generated knockout line, we observed that canonical Wnt signaling is not active, as β-catenin ablation does not alter mESC transcriptional profile in serum/LIF culture conditions. In addition, we observed that Wnt signaling activation represses mESC spontaneous differentiation in a β-catenin-dependent manner. Finally, β-catenin (ΔC) isoforms can rescue β-catenin knockout self-renewal defects in mESCs cultured in serum-free medium and, albeit transcriptionally silent, cooperate with TCF1 and LEF1 to inhibit mESC spontaneous differentiation in a GSK3-dependent manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.