ArticleProceedings of the National Academy of Sciences of the United States of America1988
Isolation of cDNAs encoding desmosomal plaque proteins: evidence that bovine desmoplakins I and II are derived from two mRNAs and a single gene.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 47 citations in OpenAlex.
- The desmoplakin tail domain position in the desmosomal plaque is isoform dependent.Journal of cell science · 2025Article
- Article
- Compound heterozygous desmoplakin mutations result in a phenotype with a combination of myocardial, skin, hair, and enamel abnormalities.The Journal of investigative dermatology · 2010Article
- Discovering the molecular components of intercellular junctions--a historical view.Cold Spring Harbor perspectives in biology · 2009Review
- Genetic alterations at the Bpag1 locus in dt mice and their impact on transcript expression.Mammalian genome : official journal of the International Mammalian Genome Society · 2005Article
- Loss of desmoplakin tail causes lethal acantholytic epidermolysis bullosa.American journal of human genetics · 2005Article
- Defining the interactions between intermediate filaments and desmosomes.The Journal of cell biology · 1998Article
- Making a connection: direct binding between keratin intermediate filaments and desmosomal proteins.The Journal of cell biology · 1994Article
- Submembranous junctional plaque proteins include potential tumor suppressor molecules.The Journal of cell biology · 1993Review
- Human autoantibodies against desmoplakins in paraneoplastic pemphigus.The Journal of clinical investigation · 1992Article
- The human gene (DSG3) coding for the pemphigus vulgaris antigen is, like the genes coding for the other two known desmogleins, assigned to chromosome 18.Human genetics · 1992Article
- Molecular structure of the human desmoplakin I and II amino terminus.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Cloning and sequence analysis of desmosomal glycoproteins 2 and 3 (desmocollins): cadherin-like desmosomal adhesion molecules with heterogeneous cytoplasmic domains.The Journal of cell biology · 1991Article
- A new high molecular mass protein showing unique localization in desmosomal plaque.The Journal of cell biology · 1989Article
- Molecular cloning and amino acid sequence of human plakoglobin, the common junctional plaque protein.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
- Perturbed intraepithelial differentiation of corneal epithelium in c-Fos-null mice.Japanese journal of ophthalmologyArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Desmoplakins (DPs) I and II (approximately equal to 240 and approximately equal to 210 kDa) are major components of the internal portion of the desmosomal cytoplasmic plaque. Desmosomes play a crucial role in cell-cell adhesion and serve as specific attachment sites for cytoplasmic intermediate filaments. Although DP-I and -II are closely related molecules, their structure (i.e., amino acid or DNA sequence) has not been determined. In addition, it is not known whether these proteins are derived from one or more genes or whether they result from posttranscriptional or posttranslational events. This paper describes the isolation and characterization of eight DP cDNA clones from a bovine lambda gt11 expression library. Fusion proteins from six of these clones selected antibodies that reacted with DP-I and -II and two selected antibodies that reacted with DP-I alone. Antibodies made against fusion protein produced by the DP1A clone reacted specifically with DP-I and -II on immunoblots. When used for indirect immunofluorescence on bovine tongue cryostat sections and cultured mouse keratinocytes, these antibodies produced a typical desmosomal staining pattern. RNA blot analysis demonstrated hybridization of three DP-I/II cDNA probes with two messages of approximately equal to 7.5 and approximately equal to 9.5 kilobases in bovine tongue RNA. In contrast, a cDNA clone that affinity-purified antibodies reacting with DP-I only hybridized exclusively with the 9.5-kilobase band. Southern blots of genomic DNA digested with a panel of restriction enzymes were hybridized with one probe derived from a DP-I/II clone and with one from a DP-I clone. Both probes hybridized with single bands of the same size in each digested sample of DNA. Together, these data suggest that DP-I and DP-II are translated from two separate messages in bovine tongue and that these messages may be derived from a single gene.
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