Evidence map›Paper›PMID 32822120›Full record

ArticleCurrent protocols in nucleic acid chemistry2020

Detection and Quantification of RNA Phosphorothioate Modifications Using Mass Spectrometry.

Ying Wu, Ya Ying Zheng, Qishan Lin, Jia Sheng

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current protocols in nucleic acid chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Ying WuDepartment of Chemistry and The RNA Institute, University at Albany, State University of New York, Albany, New York.
Ya Ying ZhengDepartment of Chemistry and The RNA Institute, University at Albany, State University of New York, Albany, New York.
Qishan LinDepartment of Chemistry and The RNA Institute, University at Albany, State University of New York, Albany, New York.
Jia ShengDepartment of Chemistry and The RNA Institute, University at Albany, State University of New York, Albany, New York.
University at Albany, State University of New York · USAlbany State University · US

Funding

SELF-SPLICING INTRONS IN THE PHAGE T4/E COLI SYSTEMR01GM039422 · NIGMS · WADSWORTH CENTER · PI BELFORT, MARLENE · 1988 to 2019
$5.3M
INTRON DYNAMICS IN BACTERIAR37GM039422 · NIGMS · WADSWORTH CENTER · PI BELFORT, MARLENE · 2001 to 2010
$4.0M
Environmental Epitranscriptomic Studies and Tools Focused on the Wobble UridineR15GM124627 · NIGMS · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI SHENG, JIA · 2017 to 2017
$443k
NIGMS NIH HHS R01 GM039422NIGMS NIH HHS R15 GM124627NIGMS NIH HHS R37 GM039422
6 · The paper itself

Abstract

This article describes a protocol for detecting and quantifying RNA phosphorothioate modifications in cellular RNA samples. Starting from solid-phase synthesis of phosphorothioate RNA dinucleotides, followed by purification with reversed-phase HPLC, phosphorothioate RNA dinucleotide standards are prepared for UPLC-MS and LC-MS/MS methods. RNA samples are extracted from cells using TRIzol reagent, then digested with a nuclease mixture and analyzed by mass spectrometry. UPLC-MS is employed first to identify RNA phosphorothioate modifications. An optimized LC-MS/MS method is then employed to quantify the frequency of RNA phosphorothioate modifications in a series of model cells. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Synthesis, purification, and characterization of RNA phosphorothioate dinucleotides Basic Protocol 2: Digestion of RNA samples extracted from cells Basic Protocol 3: Detection and quantification of RNA phosphorothioate modifications by mass spectrometry.

Indexed as

AnimalsChromatography, High Pressure LiquidChromatography, Reverse-PhaseEscherichia coliHumansLactobacillusMass SpectrometryPhosphorothioate OligonucleotidesQuality ControlReference StandardsRNASolid-Phase Synthesis TechniquesPhosphorothioate OligonucleotidesRNALC-MS/MSnucleic acidsRNA phosphorothioate modificationUPLC-MS

Identifiers

PMID32822120
PMCPMC7700719
OpenAlexW3081139221

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.