ArticleStem cells translational medicine2021
Culture substrate-associated YAP inactivation underlies chondrogenic differentiation of human induced pluripotent stem cells.
Article in Stem cells translational medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 30 citations in OpenAlex.
- Meniscal engraftment of iPSC-derived cartilage particles as hyaline and fibrous cartilage in a rabbit injury model.Regenerative therapy · 2026Article
- Exosomes of stem cells: a potential frontier in the treatment of osteoarthritis.Precision clinical medicine · 2025Review
- FAK mediates mechanical signaling to maintain epithelial homeostasis through YAP/TAZ-TEADs.Histochemistry and cell biology · 2025Article
- Suppression of YAP Ameliorates Cartilage Degeneration in Ankle Osteoarthritis via Modulation of the Wnt/β-Catenin Signaling Pathway.Calcified tissue international · 2024Article
- Biologically Relevant Laminin-511 Moderates the Derivation and Proliferation of Human Lens Epithelial Stem/Progenitor-Like Cells.Investigative ophthalmology & visual science · 2024Article
- Laminin 511 E8 fragment promotes to form basement membrane-like structure in human skin equivalents.Regenerative therapy · 2024Article
- Aspirin reverses inflammatory suppression of chondrogenesis by stabilizing YAP.Cell proliferation · 2023Article
- Expression ofFrontiers in oncology · 2023Article
- Role of YAP as a Mechanosensing Molecule in Stem Cells and Stem Cell-Derived Hematopoietic Cells.International journal of molecular sciences · 2022Review
- Generation of Monkey Induced Pluripotent Stem Cell-Derived Cartilage Lacking Major Histocompatibility Complex Class I Molecules on the Cell Surface.Tissue engineering. Part A · 2022Article
- A preview of selected articles.Stem cells translational medicine · 2021Article
- Culture substrate-associated YAP inactivation underlies chondrogenic differentiation of human induced pluripotent stem cells.Stem cells translational medicine · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
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Abstract
Human induced pluripotent stem cells (hiPSCs) are a promising cell source for the creation of cartilage to treat articular cartilage damage. The molecular mechanisms that translate culture conditions to the chondrogenic differentiation of hiPSCs remain to be analyzed. To analyze the effects of culture substrates, we chondrogenically differentiated hiPSCs on Matrigel or laminin 511-E8 while holding the composition of the chondrogenic medium constant. Cartilage was formed from hiPSCs on Matrigel, but not on laminin 511-E8. On Matrigel, the hiPSCs were round and yes-associated protein (YAP) was inactive. In contrast, on laminin 511-E8, the hiPSCs were flat and YAP was active. Treating the laminin 511-E8 hiPSCs in a bioreactor caused cell aggregates, in which the cells were round and YAP was inactive. Subsequent culture of the aggregates in chondrogenic medium resulted in cartilage formation. Transient knockdown of YAP in hiPSCs around the start of chondrogenic differentiation successfully formed cartilage on laminin 511-E8, suggesting that the activation of YAP is responsible for the failure of cartilage formation from hiPSCs on laminin 511-E8. Consistently, the addition of YAP inhibitors to laminin 511-E8 hiPSCs caused partial cartilage formation. This study contributes to identifying the molecules that mediate the effects of culture substrates on the chondrogenic differentiation of hiPSCs as well as to developing clinically applicable chondrogenic differentiation methods.
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