Evidence map›Paper›PMID 32821958›Full record

ArticleMikrochimica acta2020

A colorimetric immunoassay for determination of Escherichia coli O157:H7 based on oxidase-like activity of cobalt-based zeolitic imidazolate framework.

Shujuan Wang, Dongpo Xu, Chengchao Ding, Yachen Tian, Kangjie Ge, Liang Guo, Jie Li, Qingli Dong, Yong Huang, Qing Liu

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
  2. Review
  3. Direct, Rapid Detection of Pathogens from Urine Samples.Materials (Basel, Switzerland) · 2022
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Shujuan WangSchool of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
Dongpo XuSchool of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
Chengchao DingSchool of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
Yachen TianSchool of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
Kangjie GeSchool of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
Liang GuoSchool of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
Jie LiSchool of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
Qingli DongSchool of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
Yong HuangAffiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu University of Traditional Chinese Medicine, Sichuan, 611137, Chengdu, China. huangyong@cdutcm.edu.cn.
Qing LiuSchool of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China. liuq@usst.edu.cn.ORCID https://orcid.org/0000-0002-5152-2736
University of Shanghai for Science and Technology · CNChengdu University of Traditional Chinese Medicine · CN

Funding

National Key R&D Program of China 2018YFC1602500National Natural Science Foundation for Young Scientists of China 81502504, 31801455National Natural Science Foundation of China 81572809, 31871897Science and Technology Innovation Plan of Shanghai 18495800400
6 · The paper itself

Abstract

Cobalt-based zeolitic imidazolate framework nanosheets (ZIF-67) with oxidase-like catalytic activities as an immunoprobe were employed to enhance the sensitivity of an immunoassay. ZIF-67 was synthesized via the solvothermal method using 2-methylimidazole and cobalt dichloride as substrates. A colorimetric immunoassay for Escherichia coli (E. coli) O157:H7 was designed. Preparation of the immunoprobe involved self-polymerized dopamine being applied for the surface modification of ZIF-67 nanosheets in order to bind to the antibody, which was used to identify E. coli O157:H7. ZIF-67 catalyze the oxidation of 3,3',5,5'-tetramethylbiphenyl (TMB) and produced a color change from colorless to blue. Upon reaction termination, the absorbance was measured at 450 nm. By combining ZIF-67@PDA catalyzed chromogenic reaction with antibody recognition and magnetic separation, the limit of determination is 12 CFU mL

Indexed as

AnimalsAntibodies, ImmobilizedAntibodies, MonoclonalBreadCatalysisChromogenic CompoundsCobaltColorimetryDrinking WaterEscherichia coli O157Food ContaminationImmunoassayImmunomagnetic SeparationIndolesLimit of DetectionMagnetite NanoparticlesAntibodies, ImmobilizedAntibodies, MonoclonalChromogenic CompoundsCobaltDrinking WaterIndolesMagnetite NanoparticlesMetal-Organic FrameworkspolydopaminePolymersAntibody recognitionColorimetric immunoassayEscherichia coli O157:H7ZIF-67·dopamine self-polymerized

Identifiers

PMID32821958
OpenAlexW3080337035

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.