Evidence map›Paper›PMID 32821643›Full record

Article3 Biotech2020

Dysbiosis in intestinal microbiome linked to fecal blood determined by direct hybridization.

Concetta Cafiero, Agnese Re, Salvatore Pisconti, Marina Trombetti, Mariarita Perri, Manuela Colosimo, Gerardo D'Amato, Luca Gallelli, Roberto Cannataro, Clelia Molinario and 3 more

Abstract readCase Reports
In one paragraph

Article in 3 Biotech, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Article
  6. Cells · 2022
    Article
  7. Review
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Concetta Cafiero *Oncology Unit, SG Moscati Hospital of Taranto, Taranto, Italy.
Agnese Re *Laboratory of Medical Genetics, Alessandria Artemisia, Rome, Italy.
Salvatore PiscontiOncology Unit, SG Moscati Hospital of Taranto, Taranto, Italy.
Marina TrombettiDietetics and Aesthetic Medicine Section, Alessandria Artemisia, Rome, Italy.
Mariarita PerriDepartment of Pharmacy, Health and Nutritional Sciences-Department of Excellence 2018-2022, University of Calabria, 87036 Rende, CS Italy.
Manuela ColosimoDepartment of Microbiology and Virology, Pugliese Ciaccio Hospital, Catanzaro, Italy.
Gerardo D'AmatoDepartment of Endocrine and Metabolic Surgery, Policlinico Universitario A Gemelli-Università Cattolica del Sacro Cuore, Rome, Italy.
Luca GallelliClinical Pharmacology and Pharmacovigilance Unit, Department of Health Sciences, Mater Domini Hospital, University of Catanzaro, Catanzaro, Italy.
Roberto CannataroDepartment of Pharmacy, Health and Nutritional Sciences-Department of Excellence 2018-2022, University of Calabria, 87036 Rende, CS Italy.
Clelia MolinarioLaboratory of Medical Genetics, Alessandria Artemisia, Rome, Italy.
Alessia FazioDepartment of Pharmacy, Health and Nutritional Sciences-Department of Excellence 2018-2022, University of Calabria, 87036 Rende, CS Italy.
Maria Cristina CaroleoDepartment of Pharmacy, Health and Nutritional Sciences-Department of Excellence 2018-2022, University of Calabria, 87036 Rende, CS Italy.
Erika CioneDepartment of Pharmacy, Health and Nutritional Sciences-Department of Excellence 2018-2022, University of Calabria, 87036 Rende, CS Italy.ORCID 0000-0002-0562-0597

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The important physiological and pathophysiological roles of intestinal human microbiome (HMB) in human health have been emerging, owing to the access to molecular biology techniques. Herein we evaluated, for the first time, the intestinal HMB through direct hybridization approach using n-counter flex DX technology which bypasses the amplification procedure currently applied by other technologies to study the human microbiome. To this purpose, a clinical study was carried out on fecal samples, recruiting both healthy volunteers (N-FOB) and subjects positive for occult blood (P-FOB). A relevant custom panel of 79 16S rRNA target gene was engineered and 32 of them displayed a variation between the two clusters of subjects. Our findings revealed that bacteria belonging to Proteobacteria have higher distribution in P-FOB describing dysbiosis. Similarly, Bacteroidetes and Firmicutes phylum display high distribution in P-FOB. Of interest, the presence of

Indexed as

Clostridium difficileDirect detectionDysbiosisEubiosisMicrobiomeProteobacteria

Identifiers

PMID32821643
PMCPMC7387388

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.