ArticleJournal of the American Chemical Society2020
Assembly of a Ribozyme Ligase from Short Oligomers by Nonenzymatic Ligation.
Article in Journal of the American Chemical Society, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- A Facile Strategy for 3'-Terminal Functionalization of DNA and RNA via On-Column Conjugation with Integration into Tandem Oligonucleotide Synthesis.Current protocols · 2026Article
- Growth, Dissolution and Segregation of Genetically Encoded RNA Droplets by Ribozyme Catalysis.Angewandte Chemie (International ed. in English) · 2026Article
- Efficient Assembly of Functional RNA byJournal of the American Chemical Society · 2025Article
- A Versatile Disulfide-Containing Solid-Support Strategy for 3'-Modifiers in Oligonucleotides: Introducing Modular Tandem Oligonucleotide Synthesis.Chemistry, an Asian journal · 2025Article
- Derivatization of Solid Supports Using a Carbamate-Linked Tether Containing a Disulfide Functional Group.Current protocols · 2025Article
- Origin of the RNA World in Cold Hadean Geothermal Fields Enriched in Zinc and Potassium: Abiogenesis as a Positive Fallout from the Moon-Forming Impact?Life (Basel, Switzerland) · 2025Article
- Chemical Evolution of Life on Earth.Genes · 2025Review
- Selective Nonenzymatic Formation of Biologically Common RNA Hairpins.Angewandte Chemie (International ed. in English) · 2025Article
- Selection-diversification interplay in oligonucleotide chemical evolution.Biophysics and physicobiology · 2025Article
- A potential role for RNA aminoacylation prior to its role in peptide synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- RNA Complexes with Nicks and Gaps: Thermodynamic and Kinetic Effects of Coaxial Stacking and Dangling Ends.Journal of the American Chemical Society · 2024Article
- Constraints on the emergence of RNA through non-templated primer extension with mixtures of potentially prebiotic nucleotides.Nucleic acids research · 2024Article
- High-Fidelity RNA Copying via 2',3'-Cyclic Phosphate Ligation.Journal of the American Chemical Society · 2024Article
- Replication elongates short DNA, reduces sequence bias and develops trimer structure.Nucleic acids research · 2024Article
- The RNA-DNA world and the emergence of DNA-encoded heritable traits.RNA biology · 2024Article
- On the origin of life: an RNA-focused synthesis and narrative.RNA (New York, N.Y.) · 2023Article
- Enhanced nonenzymatic RNA copying with in-situ activation of short oligonucleotides.Nucleic acids research · 2023Article
- Minimal RNA self-reproduction discovered from a random pool of oligomers.Chemical science · 2023Article
- Experimental Tests of the Virtual Circular Genome Model for Nonenzymatic RNA Replication.Journal of the American Chemical Society · 2023Article
- Hybridization kinetics of out-of-equilibrium mixtures of short RNA oligonucleotides.Nucleic acids research · 2022Article
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Our current understanding of the chemistry of the primordial genetic material is fragmentary at best. The chemical replication of oligonucleotides long enough to perform catalytic functions is particularly problematic because of the low efficiency of nonenzymatic template copying. Here we show that this problem can be circumvented by assembling a functional ribozyme by the templated ligation of short oligonucleotides. However, this approach creates a new problem because the splint oligonucleotides used to drive ribozyme assembly strongly inhibit the resulting ribozyme. We explored three approaches to the design of splint oligonucleotides that enable efficient ligation but which allow the assembled ribozyme to remain active. DNA splints, splints with G:U wobble pairs, and splints with G to I (Inosine) substitutions all allowed for the efficient assembly of an active ribozyme ligase. Our work demonstrates the possibility of a transition from nonenzymatic ligation to enzymatic ligation and reveals the importance of avoiding ribozyme inhibition by complementary oligonucleotides.
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