ArticleThe Analyst2020
Long-term dry storage of enzyme-based reagents for isothermal nucleic acid amplification in a porous matrix for use in point-of-care diagnostic devices.
Article in The Analyst, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Heat-actuated valve implementation in a point-of-care, paper-based microfluidic device for infectious disease detection.PloS one · 2026Article
- Advances in methods for rapid detection of Staphylococcus aureus and methicillin-resistant S. aureus.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Review
- Rapid and Cost-EffectiveDiagnostics (Basel, Switzerland) · 2025Article
- A low-cost point-of-care device for the simultaneous detection of two sexually transmitted bacterial pathogens in vaginal swab samples.The Analyst · 2025Article
- Polyethylene Glycol Nanofiller for Robust Lyophilization of Graphically Encoded Hydrogel Microparticles.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Development of integrated smartphone/resistive biosensor for on-site rapid environmental monitoring of organophosphate pesticides in food and water.Biosensors & bioelectronics: X · 2023Article
- Extending the Shelf-Life of Immunoassay-Based Microfluidic Chips through Freeze-Drying Sublimation Techniques.Sensors (Basel, Switzerland) · 2023Article
- Disposable platform for bacterial lysis and nucleic acid amplification based on a single USB-powered printed circuit board.PloS one · 2023Article
- Review
- Near-digital amplification in paper improves sensitivity and speed in biplexed reactions.Scientific reports · 2022Article
- Simultaneous detection of circulating tumor DNAs using a SERS-based lateral flow assay biosensor for point-of-care diagnostics of head and neck cancer.Biomedical optics express · 2022Article
- Review
- Development of robust isothermal RNA amplification assay for lab-free testing of RNA viruses.Scientific reports · 2021Article
- Fully integrated microfluidic devices for qualitative, quantitative and digital nucleic acids testing at point of care.Biosensors & bioelectronics · 2021Review
- Review
- Dry storage of multiple reagent types within a paper microfluidic device for phenylalanine monitoring.Analytical methods : advancing methods and applications · 2021Article
- Article
- Two-Fluorophore Mobile Phone Imaging of Biplexed Real-Time NAATs Overcomes Optical Artifacts in Highly Scattering Porous Media.Analytical chemistry · 2020Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Nucleic acid amplification test (NAAT)-based point-of-care (POC) devices are rapidly growing for use in low-resource settings. However, key challenges are the ability to store the enzyme-based reagents in dry form in the device and the long-term stability of those reagents at elevated temperatures, especially where ambient temperatures could be as high as 45 °C. Here, we describe a set of excipients including a combination of trehalose, polyethylene glycol and dextran, and a method for using them that allows long-term dry storage of enzyme-based reagents for an isothermal strand displacement amplification (iSDA) reaction in a porous matrix. Various porous materials, including nitrocellulose, cellulose, and glass fiber, were tested. Co-dried reagents for iSDA always included those that amplified the ldh1 gene in Staphylococcus aureus (a polymerase and a nicking enzyme, 4 primers, dNTPs and a buffer). Reagents also either included a capture probe and a streptavidin-Au label required for lateral flow (LF) detection after amplification, or a fluorescent probe used for real-time detection. The reagents showed the best stability in a glass fiber matrix when stored in the presence of 10% trehalose and 2.5% dextran. The reagents were stable for over a year at ∼22 °C as determined by lateral flow detection and gel electrophoresis. The reagents also exhibited excellent stability after 360 h at 45 °C; the assay still detected as few as 10 copies of ldh1 gene target by lateral flow detection, and 50 copies with real-time fluorescence detection. These results demonstrate the potential for incorporation of amplification reagents in dry form in point-of-care devices for use in a wide range of settings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.