Evidence map›Paper›PMID 32820707›Full record

ArticleCirculation research2020

Gut Microbiota-Associated Activation of TLR5 Induces Apolipoprotein A1 Production in the Liver.

Jensen H C Yiu, Kam-Suen Chan, Jamie Cheung, Jin Li, Yan Liu, Yao Wang, William W L Fung, Jieling Cai, Samson W M Cheung, Bernhard Dorweiler and 4 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Circulation research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed, 1 pooled it
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 1 synthesis or guideline pooled it, 77 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Review
  5. Review
  6. Review
  7. Article
  8. Flagellin in blood andiScience · 2025
    Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. The gut microbiome as a modulator of arterial function and age-related arterial dysfunction.American journal of physiology. Heart and circulatory physiology · 2024
    Review
  14. Review
  15. Article
  16. Content of stress granules reveals a sex difference at the early phase of cold exposure in mice.American journal of physiology. Endocrinology and metabolism · 2024
    Article
  17. Article
  18. Article
  19. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 6 countries.

Jensen H C YiuState Key Laboratory of Pharmaceutical Biotechnology (J.H.C.Y., J. Cheung, J.L., Y.L., Y.W., J.Cai, S.W.M.C., A.X., C.W.W.), Li Ka Shing Faculty of Medicine, the University of Hong Kong, China.
Kam-Suen ChanDepartment of Pharmacology and Pharmacy (J.H.C.Y., K.-S.C., J. Cheung, Y.W., W.W.L.F., J. Cai, S.W.M.C., E.Y.F.W., A.X., C.W.W.), Li Ka Shing Faculty of Medicine, the University of Hong Kong, China.
Jamie CheungState Key Laboratory of Pharmaceutical Biotechnology (J.H.C.Y., J. Cheung, J.L., Y.L., Y.W., J.Cai, S.W.M.C., A.X., C.W.W.), Li Ka Shing Faculty of Medicine, the University of Hong Kong, China.
Jin LiState Key Laboratory of Pharmaceutical Biotechnology (J.H.C.Y., J. Cheung, J.L., Y.L., Y.W., J.Cai, S.W.M.C., A.X., C.W.W.), Li Ka Shing Faculty of Medicine, the University of Hong Kong, China.
Yan LiuState Key Laboratory of Pharmaceutical Biotechnology (J.H.C.Y., J. Cheung, J.L., Y.L., Y.W., J.Cai, S.W.M.C., A.X., C.W.W.), Li Ka Shing Faculty of Medicine, the University of Hong Kong, China.
Yao WangState Key Laboratory of Pharmaceutical Biotechnology (J.H.C.Y., J. Cheung, J.L., Y.L., Y.W., J.Cai, S.W.M.C., A.X., C.W.W.), Li Ka Shing Faculty of Medicine, the University of Hong Kong, China.
William W L FungDepartment of Pharmacology and Pharmacy (J.H.C.Y., K.-S.C., J. Cheung, Y.W., W.W.L.F., J. Cai, S.W.M.C., E.Y.F.W., A.X., C.W.W.), Li Ka Shing Faculty of Medicine, the University of Hong Kong, China.
Jieling CaiDepartment of Pharmacology and Pharmacy (J.H.C.Y., K.-S.C., J. Cheung, Y.W., W.W.L.F., J. Cai, S.W.M.C., E.Y.F.W., A.X., C.W.W.), Li Ka Shing Faculty of Medicine, the University of Hong Kong, China.
Samson W M CheungState Key Laboratory of Pharmaceutical Biotechnology (J.H.C.Y., J. Cheung, J.L., Y.L., Y.W., J.Cai, S.W.M.C., A.X., C.W.W.), Li Ka Shing Faculty of Medicine, the University of Hong Kong, China.
Bernhard DorweilerDepartment of Vascular and Endovascular Surgery, University Hospital Cologne, Germany (B.D.).
Eric Y F WanDepartment of Pharmacology and Pharmacy (J.H.C.Y., K.-S.C., J. Cheung, Y.W., W.W.L.F., J. Cai, S.W.M.C., E.Y.F.W., A.X., C.W.W.), Li Ka Shing Faculty of Medicine, the University of Hong Kong, China.
Patrick TsoMetabolic Phenotyping Center, Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, OH (P.T.).
Aimin XuState Key Laboratory of Pharmaceutical Biotechnology (J.H.C.Y., J. Cheung, J.L., Y.L., Y.W., J.Cai, S.W.M.C., A.X., C.W.W.), Li Ka Shing Faculty of Medicine, the University of Hong Kong, China.
Connie W WooState Key Laboratory of Pharmaceutical Biotechnology (J.H.C.Y., J. Cheung, J.L., Y.L., Y.W., J.Cai, S.W.M.C., A.X., C.W.W.), Li Ka Shing Faculty of Medicine, the University of Hong Kong, China.
World Wide Web Consortium · USPharmaceutical Biotechnology (Czechia) · CZShanxi Medical University · CNUniversity Hospital Cologne · DEUniversity of Cincinnati Medical Center · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

rationaleDysbiosis of gut microbiota plays an important role in cardiovascular diseases but the molecular mechanisms are complex. An association between gut microbiome and the variance in HDL-C (high-density lipoprotein-cholesterol) level was suggested in a human study. Besides, dietary fat was shown to increase both HDL-C and LDL-C (low-density lipoprotein-cholesterol) levels. We speculate that certain types of gut bacteria responding to dietary fat may help to regulate HDL-C level and potentially affect atherosclerotic development.

objectiveWe aimed to investigate whether and how high-fat diet (HFD)-associated gut microbiota regulated HDL-C level. METHODS AND

resultsWe found that HFD increased gut flagellated bacteria population in mice. The increase in HDL-C level was adopted by mice receiving fecal microbiome transplantation from HFD-fed mouse donors. HFD led to increased hepatic but not circulating flagellin, and deletion of TLR5 (Toll-like receptor 5), a receptor sensing flagellin, suppressed HFD-stimulated HDL-C and ApoA1 (apolipoprotein A1) levels. Overexpression of TLR5 in the liver of TLR5-knockout mice was able to partially restore the production of ApoA1 and HDL-C levels. Mechanistically, TLR5 activation by flagellin in primary hepatocytes stimulated ApoA1 production through the transcriptional activation responding to the binding of NF-κB (nuclear factor-κB) on

conclusionsOur finding suggests that commensal flagellated bacteria in gut can facilitate ApoA1 and HDL-C productions in liver through activation of TLR5 in hepatocytes. Hepatic TLR5 may be a potential drug target to increase ApoA1.

Indexed as

Gastrointestinal MicrobiomeAnimalsApolipoprotein A-ICholesterol, HDLDietary FatsFlagellinLiverMiceNF-kappa BToll-Like Receptor 5Apolipoprotein A-ICholesterol, HDLDietary FatsFlagellinNF-kappa BTLR5 protein, humanToll-Like Receptor 5apolipoproteinsatherosclerosisgastrointestinal microbiomehepatocyteslipoproteinspharmacologyToll-like receptors

Identifiers

PMID32820707
PMCPMC7580858
OpenAlexW3081115326

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.