Evidence map›Paper›PMID 32820422›Full record

ArticleNeuromolecular medicine2020

Cell-Free Extracellular Vesicles Derived from Human Bone Marrow Endothelial Progenitor Cells as Potential Therapeutics for Microvascular Endothelium Restoration in ALS.

Svitlana Garbuzova-Davis, Alison E Willing, Jared Ehrhart, Lianchun Wang, Paul R Sanberg, Cesario V Borlongan

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuromolecular medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 41 citations in OpenAlex.

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  20. Treating Metastatic Brain Cancers With Stem Cells.Frontiers in molecular neuroscience · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Svitlana Garbuzova-DavisCenter of Excellence for Aging & Brain Repair, Morsani College of Medicine, University of South Florida, Tampa, FL, USA. sgarbuzo@usf.edu.ORCID 0000-0001-5816-0937
Alison E WillingCenter of Excellence for Aging & Brain Repair, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.
Jared EhrhartCenter of Excellence for Aging & Brain Repair, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.
Lianchun WangDepartment of Molecular Pharmacology and Physiology, Byrd Alzheimer`S Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.
Paul R SanbergCenter of Excellence for Aging & Brain Repair, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.
Cesario V BorlonganCenter of Excellence for Aging & Brain Repair, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.
University of South Florida · US

Funding

Robo4 and heparan sulfate proteoglycan in angiogenesisR01HL093339 · NHLBI · UNIVERSITY OF SOUTH FLORIDA · PI WANG, LIANCHUN · 2009 to 2018
$3.4M
Heparan sulfate proteoglycan in the brain vascular clearance of amyloid-β and Alzheimer's diseaseRF1AG074289 · NIA · UNIVERSITY OF SOUTH FLORIDA · PI WANG, LIANCHUN · 2021 to 2021
$1.8M
Re-Establishing Vascular Integrity in ALS via Endothelial Cell TransplantR01NS090962 · NINDS · UNIVERSITY OF SOUTH FLORIDA · PI BORLONGAN, CESARIO V, GARBUZOVA-DAVIS, SVITLANA NICOLAI · 2015 to 2019
$1.6M
NHLBI NIH HHS R01 HL093339NIA NIH HHS RF1 AG074289NINDS NIH HHS R01 NS090962
6 · The paper itself

Abstract

Repairing the damaged blood-CNS-barrier in amyotrophic lateral sclerosis (ALS) is necessary to prevent entry of detrimental blood-borne factors contributing to motor neuron dysfunction. Recently, we showed benefits of human bone marrow endothelial progenitor cell (hBM-EPC) transplantation into symptomatic ALS mice on barrier restoration by replacing damaged endothelial cells (ECs). Additionally, transplanted cells may endogenously repair ECs by secreting angiogenic factors as our subsequent in vitro study demonstrated. Based on these study results, hBM-EPCs may secrete extracellular vesicles, which may contain and transfer diverse vesicular biomolecules towards maintenance of EC functionality. The study aimed to characterize extracellular vesicles (EVs) derived from hBM-EPCs as potential cell-free therapeutics for endothelium repair in ALS. EVs were isolated from hBM-EPC media at different culture times and vesicle properties were evaluated. The protective effects of EVs on mouse brain endothelial cells (mBECs) exposed to ALS mouse plasma were investigated. Uptake and blockage of EVs from GFP-transfected hBM-EPCs in ECs were determined in vitro. Results showed that EVs isolated from hBM-EPCs as nanosized vesicles significantly reduced mBEC damage from the pathological environment and these EVs were taken up by cells. Blockage of β1 integrin on EVs prevented internalization of vesicles in mBECs. Together, these results provide evidence for potential of hBM-EPC-derived EVs as novel cell-free therapeutics for repair of endothelium in ALS. Although determining translational potential of hBM-EPC-derived EVs will require evaluation in vivo, this in vitro study represents a step towards an extracellular vesicle-based approach for repair of the damaged microvascular endothelium in ALS.

Indexed as

Amyotrophic Lateral SclerosisAnimalsBlood-Brain BarrierBone Marrow CellsCells, CulturedCulture Media, ConditionedDisease Models, AnimalEndothelial Progenitor CellsEndothelium, VascularExtracellular VesiclesGenes, ReporterHumansMaleMiceSuperoxide Dismutase-1Culture Media, ConditionedSod1 protein, mouseSuperoxide Dismutase-1ALS mouse plasmaExtracellular vesiclesHuman bone marrow endothelial progenitor cellsIn vitroMouse brain endothelial cell line

Identifiers

PMID32820422
PMCPMC7677172
OpenAlexW3080377352

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.