ArticleCommunications biology2020
A droplet reactor on a super-hydrophobic surface allows control and characterization of amyloid fibril growth.
Article in Communications biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Antenna-enhanced mid-infrared detection of extracellular vesicles derived from human cancer cell cultures.Journal of nanobiotechnology · 2022Article
- Wall-free droplet microfluidics for probing biological processes by high-brilliance X-ray scattering techniques.Frontiers in molecular biosciences · 2022Review
- Micro/Nanopatterned Superhydrophobic Surfaces Fabrication for Biomolecules and Biomaterials Manipulation and Analysis.Micromachines · 2021Review
- DNA Studies: Latest Spectroscopic and Structural Approaches.Micromachines · 2021Review
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Authors and funding
18 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Methods to produce protein amyloid fibrils, in vitro, and in situ structure characterization, are of primary importance in biology, medicine, and pharmacology. We first demonstrated the droplet on a super-hydrophobic substrate as the reactor to produce protein amyloid fibrils with real-time monitoring of the growth process by using combined light-sheet microscopy and thermal imaging. The molecular structures were characterized by Raman spectroscopy, X-ray diffraction and X-ray scattering. We demonstrated that the convective flow induced by the temperature gradient of the sample is the main driving force in the growth of well-ordered protein fibrils. Particular attention was devoted to PHF6 peptide and full-length Tau441 protein to form amyloid fibrils. By a combined experimental with the molecular dynamics simulations, the conformational polymorphism of these amyloid fibrils were characterized. The study provided a feasible procedure to optimize the amyloid fibrils formation and characterizations of other types of proteins in future studies.
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