Evidence map›Paper›PMID 32819579›Full record

ArticleBiochemical and biophysical research communications2020

Probing the unfolded protein response in long-lived naked mole-rats.

Zhen Du, Sampurna Chakrabarti, Yavuz Kulaberoglu, Ewan St John Smith, Christopher M Dobson, Laura S Itzhaki, Janet R Kumita

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical and biophysical research communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. DNA Homeostasis and Senescence: Lessons from the Naked Mole Rat.International journal of molecular sciences · 2021
    Review
  5. Regulation of Age-Related Protein Toxicity.Frontiers in cell and developmental biology · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Zhen DuDepartment of Pharmacology, University of Cambridge, Tennis Court Rd., Cambridge, CB2 1PD, UK; Centre for Misfolding Diseases, Department of Chemistry, University of Cambridge, Lensfield Rd., Cambridge, CB2 1EW, UK.
Sampurna ChakrabartiDepartment of Pharmacology, University of Cambridge, Tennis Court Rd., Cambridge, CB2 1PD, UK.
Yavuz KulaberogluDepartment of Pharmacology, University of Cambridge, Tennis Court Rd., Cambridge, CB2 1PD, UK; UCL Institute of Healthy Ageing, Darwin Building, 104 Gower St, Bloomsbury, London, WC1E 6AD, UK.
Ewan St John SmithDepartment of Pharmacology, University of Cambridge, Tennis Court Rd., Cambridge, CB2 1PD, UK.
Christopher M DobsonCentre for Misfolding Diseases, Department of Chemistry, University of Cambridge, Lensfield Rd., Cambridge, CB2 1EW, UK.
Laura S ItzhakiDepartment of Pharmacology, University of Cambridge, Tennis Court Rd., Cambridge, CB2 1PD, UK. Electronic address: lsi10@cam.ac.uk.
Janet R KumitaDepartment of Pharmacology, University of Cambridge, Tennis Court Rd., Cambridge, CB2 1PD, UK; Centre for Misfolding Diseases, Department of Chemistry, University of Cambridge, Lensfield Rd., Cambridge, CB2 1EW, UK. Electronic address: jrk38@cam.ac.uk.
University of Cambridge · GB

Funding

Cancer Research UK 22053Cancer Research UK C56829/A22053Wellcome Trust 094425/Z/10/Z
6 · The paper itself

Abstract

The long-living naked mole-rat (NMR) shows negligible senescence and resistance to age-associated diseases. Recent evidence, based on protein-level assays, suggests that enhanced protein homeostasis machinery contributes to NMR stress-resistance and longevity. Here, we develop NMR-specific, transcriptional assays for measuring the unfolded protein response (UPR), a component of ER proteostasis. By varying doses and response times of pharmacological ER stressors applied to NMR kidney fibroblasts, we probe the NMR UPR in detail, demonstrating that NMR fibroblasts have a higher UPR activation threshold compared to mouse fibroblasts under mild ER-stress induction; whereas temporal analysis reveals that severe ER-stress induction results in no comparative differences. Probing NMR UPR activation with our robust assays may lead to insights into the proteostasis and ageing relationship.

Indexed as

LongevityUnfolded Protein ResponseAnimalsApoptosisCells, CulturedEndoplasmic ReticulumEndoplasmic Reticulum-Associated DegradationEndoplasmic Reticulum StressFemaleFibroblastsGene Expression RegulationKidneyMaleMiceMole RatsProtein FoldingProtein Serine-Threonine KinasesX-Box Binding Protein 1AgeingER stressNaked mole-ratProtein homeostasisUnfolded protein response

Identifiers

PMID32819579
PMCPMC7453385
OpenAlexW3046988184

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.