ArticleBasic and clinical andrology2020
Could high DNA stainability (HDS) be a valuable indicator of sperm nuclear integrity?
Article in Basic and clinical andrology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- Meta-correlation of Sperm Morphology and DNA Fragmentation Index.Reproductive sciences (Thousand Oaks, Calif.) · 2025Pooled it
- Alpha-Lipoic Acid Supplementation for Male Partner of Couples with Recurrent Pregnancy Loss: A Post hoc analysis in Clinical Trial.International journal of fertility & sterility · 2023Trial
- Assessment of Associations Between Demographic Characteristics, Lifestyle Factors, and Sperm Quality Among Men Presenting With Infertility Complaints.Health science reports · 2026Article
- Cryopreservation of Aseel rooster sperm using different glycerol-iodixanol combinations and evaluation of post-thaw quality.BMC veterinary research · 2026Article
- Integrating Sperm Microscopy, Environmental Exposures, and Lifestyle Factors for Male Fertility Analysis: Protocol for the Nippon Semen and Environmental Exposure Database (N-SEED) Cross-Sectional Study.JMIR research protocols · 2026Article
- The role of sperm DNA fragmentation testing in predicting IVF and PGT-A outcomes.Scientific reports · 2026Article
- Separation of sperm based on rheotaxis mechanism using a microfluidic device.Clinical and experimental reproductive medicine · 2026Article
- Effects of long-term cryopreservation on sperm quality and oxidative stress in Manchega rams (Ovis aries).Reproduction (Cambridge, England) · 2025Article
- Induction of Sperm DNA Fragmentation by Cryopreservation and In Vitro Incubation: Comparison of TUNEL, SCSA, SCD Test and COMET Assay.International journal of molecular sciences · 2025Article
- Longitudinal trends and correlation between autism spectrum disorder prevalence and sperm quality parameters (2000-2024): a comprehensive statistical analysis.Frontiers in reproductive health · 2024Article
- Sperm chromatin structure assay (SCSABasic and clinical andrology · 2023Article
- Analysis of sperm chromatin packaging and reproductive biomarker to evaluate the consequence of advanced male age.Frontiers in endocrinology · 2023Article
- Article
- Seminal Plasma Protein N-Glycan Peaks Are Potential Predictors of Semen Pathology and Sperm Chromatin Maturity in Men.Life (Basel, Switzerland) · 2021Article
- Direct but Not Indirect Methods Correlate the Percentages of Sperm With Altered Chromatin to the Intensity of Chromatin Damage.Frontiers in veterinary science · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Sperm Chromatin Structure Assay (SCSA®), in addition to identifying the DNA Fragmentation Index (DFI) also identifies High DNA satiability (HDS), supposed to reflect the nuclear compaction of spermatozoa. However, data on what exactly this parameter reveals, its relevance and usefulness are contradictory. In order to shed light on this situation, spermatozoa of a cohort (
resultsHDS does not appear to be a reliable indicator of nuclear immaturity because it shows a weak correlation with the CMA3, AB and TB stains. The low correlation of HDS with sperm DNA fragmentation (TUNEL and SCSA®) and DNA condensation (CMA3, AB and TB) tests suggests that these two parameters could be decoupled. Unlike DFI and TUNEL, HDS has not been shown to correlate with classic clinical situations of male infertility (asthenozoospermia, teratozoospermia or astheno-teratozoospermia).
conclusionHDS correlates poorly with most tests that focus specifically on the level of maturity of the sperm nucleus. To our knowledge, this study is the first to compare SCSA®, TUNEL, AB, TB and CMA3 assays on identical samples. It shows the potency, consistency and limitations of each test and the care that must be taken in their interpretation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.