Evidence map›Paper›PMID 32817529›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2020

NusG controls transcription pausing and RNA polymerase translocation throughout the

Alexander V Yakhnin, Peter C FitzGerald, Carl McIntosh, Helen Yakhnin, Maria Kireeva, Joshua Turek-Herman, Zachary F Mandell, Mikhail Kashlev, Paul Babitzke

Open access · greenAbstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 51 citations in OpenAlex.

  1. Article
  2. RNAP-seq:bioRxiv : the preprint server for biology · 2026
    Article
  3. mBio · 2025
    Article
  4. Article
  5. Review
  6. Review
  7. Review
  8. Article
  9. Article
  10. Review
  11. Article
  12. Review
  13. Article
  14. Review
  15. Article
  16. Article
  17. Robust regulation of transcription pausing inProceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  18. Article
  19. Allosteric mechanism of transcription inhibition by NusG-dependent pausing of RNA polymerase.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Alexander V YakhninDepartment of Biochemistry and Molecular Biology, Center for RNA Molecular Biology, Pennsylvania State University, University Park, PA 16802.ORCID 0000-0002-7313-7054
Peter C FitzGeraldGenome Analysis Unit, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892.
Carl McIntoshGenome Analysis Unit, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892.
Helen YakhninDepartment of Biochemistry and Molecular Biology, Center for RNA Molecular Biology, Pennsylvania State University, University Park, PA 16802.ORCID 0000-0002-7865-7112
Maria KireevaRNA Biology Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702.
Joshua Turek-HermanRNA Biology Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702.
Zachary F MandellDepartment of Biochemistry and Molecular Biology, Center for RNA Molecular Biology, Pennsylvania State University, University Park, PA 16802.
Mikhail KashlevRNA Biology Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702; kashlevm@mail.nih.gov pxb28@psu.edu.ORCID 0000-0002-1260-6486
Paul BabitzkeDepartment of Biochemistry and Molecular Biology, Center for RNA Molecular Biology, Pennsylvania State University, University Park, PA 16802; kashlevm@mail.nih.gov pxb28@psu.edu.ORCID 0000-0003-2481-1062
National Cancer Institute · USPennsylvania State University · US

Funding

Mechanisms of transcription pausing and fidelity in prokaryotes and eukaryotesZIABC010795 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI KASHLEV, MIKHAIL · 2009 to 2025
$18.1M
Mechanism of transcription-coupled DNA repair and transcription mutagenesisZIABC011202 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI KASHLEV, MIKHAIL · 2009 to 2020
$4.5M
Regulation of transcription elongationR01GM098399 · NIGMS · PENNSYLVANIA STATE UNIVERSITY, THE · PI BABITZKE, PAUL L · 2012 to 2024
$3.6M
NIGMS NIH HHS R01 GM098399
6 · The paper itself

Abstract

Transcription is punctuated by RNA polymerase (RNAP) pausing. These pauses provide time for diverse regulatory events that can modulate gene expression. Transcription elongation factors dramatically affect RNAP pausing in vitro, but the genome-wide role of such factors on pausing has not been examined. Using native elongating transcript sequencing followed by RNase digestion (RNET-seq), we analyzed RNAP pausing in

Indexed as

Bacillus subtilisBacterial ProteinsDNA-Directed RNA PolymerasesEscherichia coli ProteinsGene Expression Regulation, BacterialNucleic Acid ConformationOperonPeptide Elongation FactorsTranscriptional Elongation FactorsTranscription FactorsTranscription, GeneticTranslocation, GeneticBacterial ProteinsDNA-Directed RNA PolymerasesEscherichia coli ProteinsNusG protein, E coliPeptide Elongation FactorsTranscriptional Elongation FactorsTranscription FactorsNusGriboswitchRNA polymerase pausingRNET-seqtranslocation register

Identifiers

PMID32817529
PMCPMC7474616
OpenAlexW3067081823

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.