ArticleProceedings of the National Academy of Sciences of the United States of America2020
Single-molecule diffusometry reveals no catalysis-induced diffusion enhancement of alkaline phosphatase as proposed by FCS experiments.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 52 citations in OpenAlex.
- Enhanced Diffusion of Single, Lipid-Tethered Enzymes.Nano letters · 2026Article
- Super-Resolution Mapping and Quantification of Molecular Diffusion via Single-Molecule Displacement/Diffusivity Mapping (SMAccounts of chemical research · 2025Article
- Force Generation by Enhanced Diffusion in Enzyme-Loaded Vesicles.Nano letters · 2025Article
- Impact of Ligand-Induced Oligomer Dissociation on Enzyme Diffusion, Directly Observed at the Single-Molecule Level.Nano letters · 2025Article
- Single molecule-driven nanomotors reveal the dynamic-disordered chemomechanical transduction of active enzymes.Science advances · 2025Article
- Macromolecular Crowding, Phase Separation, and Homeostasis in the Orchestration of Bacterial Cellular Functions.Chemical reviews · 2024Review
- Motility of an autonomous protein-based artificial motor that operates via a burnt-bridge principle.Nature communications · 2024Article
- Mechanism of ADP-Inhibited ATP Hydrolysis in Single Proton-Pumping FInternational journal of molecular sciences · 2023Article
- Phase-separating pyrenoid proteins form complexes in the dilute phase.Communications biology · 2023Article
- Perspective: a stirring role for metabolism in cells.Molecular systems biology · 2022Review
- Displacement Statistics of Unhindered Single Molecules Show no Enhanced Diffusion in Enzymatic Reactions.Journal of the American Chemical Society · 2022Article
- Molecular Diffusivity of Click Reaction Components: The Diffusion Enhancement Question.Journal of the American Chemical Society · 2022Article
- Nanopore electro-osmotic trap for the label-free study of single proteins and their conformations.Nature nanotechnology · 2021Article
- Nanoscale Catalyst Chemotaxis Can Drive the Assembly of Functional Pathways.The journal of physical chemistry. B · 2021Article
- Chemically-powered swimming and diffusion in the microscopic world.Nature reviews. Chemistry · 2021Review
- A thermodynamic perspective on enhanced enzyme diffusion.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Master curve of boosted diffusion for 10 catalytic enzymes.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Theoretical and experimental observations that catalysis enhances the diffusion of enzymes have generated exciting implications about nanoscale energy flow, molecular chemotaxis, and self-powered nanomachines. However, contradictory claims on the origin, magnitude, and consequence of this phenomenon continue to arise. To date, experimental observations of catalysis-enhanced enzyme diffusion have relied almost exclusively on fluorescence correlation spectroscopy (FCS), a technique that provides only indirect, ensemble-averaged measurements of diffusion behavior. Here, using an anti-Brownian electrokinetic (ABEL) trap and in-solution single-particle tracking, we show that catalysis does not increase the diffusion of alkaline phosphatase (ALP) at the single-molecule level, in sharp contrast to the ∼20% enhancement seen in parallel FCS experiments using
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