ArticleProceedings of the National Academy of Sciences of the United States of America2020
Synthetic conversion of leaf chloroplasts into carotenoid-rich plastids reveals mechanistic basis of natural chromoplast development.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 130 citations in OpenAlex.
- Metabolic cues are the main drivers for the differentiation of chloroplasts into chromoplasts and other plastid types.Journal of experimental botany · 2026Review
- Chemical modulation of chloroplast de- and redifferentiation reveals a role for the SAL1-PAP retrograde pathway in facilitating plastid transitions.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- ORANGE: a tale of two pigments and two organelles.Plant cell reports · 2026Review
- Pro-Vitamin A Biofortified Cavendish Banana: Trait Stability in the Field.Plant biotechnology journal · 2026Article
- Carotenoid aggregates negatively impact chlorophyll levels and disrupt chloroplast development in peaches.Molecular horticulture · 2026Article
- A Chemical Probe for Increasing Leaf Tocopherol Levels by Coordinated Modulation of Biosynthesis, Competition and Storage.Plant biotechnology journal · 2026Article
- Tomato lipocalins mediate ABA (abscisic acid)- and ethylene-dependent regulation of stress tolerance and fruit ripening.Plant biotechnology (Tokyo, Japan) · 2026Article
- Regulation of chloroplast biogenesis and differentiation.Journal of experimental botany · 2026Review
- The carotenoids metabolism during tomato fruit ripening: insights into multi-level regulatory network.BMC plant biology · 2026Review
- Microbial conversion of biodiesel waste for carotenoid production.Frontiers in bioengineering and biotechnology · 2026Article
- Chloroplast genomes of nineFrontiers in plant science · 2026Article
- Plant plastids: from evolutionary origins to functional specialization and organelle interactions.Journal of experimental botany · 2025Review
- Chemical modulation of chloroplast de- and re-differentiation reveals a role for the SAL1-PAP retrograde pathway in facilitating plastid transitions.bioRxiv : the preprint server for biology · 2025Article
- Novel plant cell suspension platforms for saffron apocarotenoid production and its impact on carotenoid and volatile profiles.Plant biotechnology journal · 2025Article
- Maize leaf yellowing gene ZmCAAX modulates growth and drought resistance by regulating abscisic acid contents through interaction with the ABA biosynthetic enzyme ZmNCED3.Plant biotechnology journal · 2025Article
- High-efficiency leucoplast transit peptides for manipulating plastid protein production.Nature plants · 2025Article
- Comparative Analysis of Plastomes ofGenes · 2025Article
- Altitude-Dependent Morphophysiological, Anatomical, and Metabolomic Adaptations inPlants (Basel, Switzerland) · 2024Article
- Comparative Chloroplast Genomes Analysis Provided Adaptive Evolution Insights inInternational journal of molecular sciences · 2024Article
- Research progress on differentiation and regulation of plant chromoplasts.Molecular biology reports · 2024Review
Corrections and comments
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Authors and funding
15 authors at 7 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plastids, the defining organelles of plant cells, undergo physiological and morphological changes to fulfill distinct biological functions. In particular, the differentiation of chloroplasts into chromoplasts results in an enhanced storage capacity for carotenoids with industrial and nutritional value such as beta-carotene (provitamin A). Here, we show that synthetically inducing a burst in the production of phytoene, the first committed intermediate of the carotenoid pathway, elicits an artificial chloroplast-to-chromoplast differentiation in leaves. Phytoene overproduction initially interferes with photosynthesis, acting as a metabolic threshold switch mechanism that weakens chloroplast identity. In a second stage, phytoene conversion into downstream carotenoids is required for the differentiation of chromoplasts, a process that involves a concurrent reprogramming of nuclear gene expression and plastid morphology for improved carotenoid storage. We hence demonstrate that loss of photosynthetic competence and enhanced production of carotenoids are not just consequences but requirements for chloroplasts to differentiate into chromoplasts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.