ArticleThe Journal of biological chemistry2020
Avian eggshell formation reveals a new paradigm for vertebrate mineralization via vesicular amorphous calcium carbonate.
Article in The Journal of biological chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- Comparative Assessment of Methods for Isolation and Imaging of Extracellular Vesicles.Journal of extracellular biology · 2026Article
- Dietary Glucosamine: Effects and Mechanisms in Relation to Production Performance, Eggshell Quality, and Liver Health of Aged Laying Hens.Animals : an open access journal from MDPI · 2026Article
- Optimizing eggshell quality in extended laying periods: Insights and strategies.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Review
- An Optimized Positive Staining Protocol Method for Clear Visualization of Extracellular Vesicles by TEM Using Uranyless and Lead Citrate.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- The Role of Confinement in Biomineralization.Chemical reviews · 2025Review
- Research Progress on Genetic Factors of Poultry Egg Quality: A Review.Animals : an open access journal from MDPI · 2025Review
- Integrated mRNA-seq and miRNA-seq analysis reveals miR-210a-5p regulates uterine aging in laying hens by targeting the RASL11B/Raf/MAPK pathway.Journal of animal science and biotechnology · 2025Article
- Functional Biomaterials Derived from Protein Liquid-Liquid Phase Separation and Liquid-to-Solid Transition.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Structural Insights into Ratite Birds and Crocodile Eggshells for Advanced Biomaterials Design.ACS omega · 2025Article
- Bio-Informed Porous Mineral-Based Composites.Small (Weinheim an der Bergstrasse, Germany) · 2025Review
- Plasma Biochemistry, Intestinal Health, and Transcriptome Analysis Reveal Why Laying Hens Produce Translucent Eggs.Animals : an open access journal from MDPI · 2024Article
- Molecular genetic foundation of a sex-linked tailless trait in Hongshan chicken by whole genome data analysis.Poultry science · 2024Article
- Microbially Induced Calcium Carbonate Precipitation byApplied and environmental microbiology · 2023Review
- Evidence for heterothermic endothermy and reptile-like eggshell mineralization inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Adjusting phosphate feeding regimen according to daily rhythm increases eggshell quality via enhancing medullary bone remodeling in laying hens.Journal of animal science and biotechnology · 2023Article
- Effects of Selenium Yeast on Egg Quality, Plasma Antioxidants, Selenium Deposition and Eggshell Formation in Aged Laying Hens.Animals : an open access journal from MDPI · 2023Article
- Physiological regulation of calcium and phosphorus utilization in laying hens.Frontiers in physiology · 2023Review
- First titanosaur dinosaur nesting site from the Late Cretaceous of Brazil.Scientific reports · 2022Article
- Proteomic Analysis of Chicken Chorioallantoic Membrane (CAM) during Embryonic Development Provides Functional Insight.BioMed research international · 2022Article
- Liquid-Liquid Phase Separation in Nucleation Process of Biomineralization.Frontiers in chemistry · 2022Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Amorphous calcium carbonate (ACC) is an unstable mineral phase, which is progressively transformed into aragonite or calcite in biomineralization of marine invertebrate shells or avian eggshells, respectively. We have previously proposed a model of vesicular transport to provide stabilized ACC in chicken uterine fluid where eggshell mineralization takes place. Herein, we report further experimental support for this model. We confirmed the presence of extracellular vesicles (EVs) using transmission EM and showed high levels of mRNA of vesicular markers in the oviduct segments where eggshell mineralization occurs. We also demonstrate that EVs contain ACC in uterine fluid using spectroscopic analysis. Moreover, proteomics and immunofluorescence confirmed the presence of major vesicular, mineralization-specific and eggshell matrix proteins in the uterus and in purified EVs. We propose a comprehensive role for EVs in eggshell mineralization, in which annexins transfer calcium into vesicles and carbonic anhydrase 4 catalyzes the formation of bicarbonate ions (HCO[Formula: see text]), for accumulation of ACC in vesicles. We hypothesize that ACC is stabilized by ovalbumin and/or lysozyme or additional vesicle proteins identified in this study. Finally, EDIL3 and MFGE8 are proposed to serve as guidance molecules to target EVs to the mineralization site. We therefore report for the first-time experimental evidence for the components of vesicular transport to supply ACC in a vertebrate model of biomineralization.
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