ArticleThe Biochemical journal1988
Effects of a phorbol ester and clomiphene on protein phosphorylation and insulin secretion in rat pancreatic islets.
Article in The Biochemical journal, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 31 citations in OpenAlex.
- Regulation of insulin exocytosis by calcium-dependent protein kinase C in beta cells.Cell calcium · 2017Review
- Phorbol ester impairs electrical excitation of rat pancreatic beta-cells through PKC-independent activation of KATP channels.BMC pharmacology · 2001Article
- Increase in insulin release from rat pancreatic islets by quinolone antibiotics.British journal of pharmacology · 1996Article
- Protein phosphorylation and beta-cell function.Diabetologia · 1994Review
- The role of reduced glucose transporter content and glucose metabolism in the immature secretory responses of fetal rat pancreatic islets.Diabetologia · 1994Article
- Inhibition of protein-kinase-C--dependent cell proliferation of human lung cancer cell lines by the dihydropyridine dexniguldipine.Journal of cancer research and clinical oncology · 1994Article
- The role of cytosolic free Ca2+ and protein kinase C in acetylcholine-induced insulin release in the clonal beta-cell line, HIT-T15.The Biochemical journal · 1990Article
- Phorbol-ester-induced down-regulation of protein kinase C in mouse pancreatic islets. Potentiation of phase 1 and inhibition of phase 2 of glucose-induced insulin secretion.The Biochemical journal · 1990Article
- Effects of protein kinase C activation on the regulation of the stimulus-secretion coupling in pancreatic beta-cells.The Biochemical journal · 1989Article
- Comparison of effects of phorbol esters and glucose on protein kinase C activation and insulin secretion in pancreatic islets.The Biochemical journal · 1989Article
- The effect of monooleoylglycerol on insulin secretion from isolated perifused rat islets.Diabetologia · 1989Article
- Protein phosphorylation in electrically permeabilized islets of Langerhans. Effects of Ca2+, cyclic AMP, a phorbol ester and noradrenaline.The Biochemical journal · 1988Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The potentiation of glucose-stimulated insulin release induced by 100 nM-12-O-tetradecanoylphorbol 13-acetate (TPA) was inhibited by clomiphene, an inhibitor of protein kinase C (PK C), in a dose-dependent manner. Clomiphene at concentrations up to 50 microM had a modest inhibitory action (27%) on insulin release stimulated by 10 mM-glucose alone, but had no effect on the potentiation of insulin release induced by forskolin. Islet PK C activity, associated with a particulate fraction, was stimulated maximally by 100 nM-TPA. This stimulation was blocked by clomiphene in a dose-dependent manner, with 50% inhibition at 30 microM. Incubation of intact islets with TPA after preincubation with [32P]Pi and 10 mM-glucose to label intracellular ATP resulted primarily in enhanced phosphorylation of a 37 kDa protein (mean value, +/- S.E.M., 36,700 +/- 600 Da; n = 7). This increased phosphorylation was blocked by the simultaneous inclusion of clomiphene. Subcellular fractionation revealed the presence of the 37 kDa phosphoprotein in a 24,000 g particulate fraction of islet homogenates. Neither clomiphene nor TPA affected the rate of glucose oxidation by islets. These results show that the phosphorylation state of a 37 kDa membrane protein parallels the modulation of insulin release induced by TPA and clomiphene and support a role for PK C in the insulin-secretory mechanism.
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