ArticleBioTechniques2020
A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates.
Article in BioTechniques, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Messenger RNA-encoded antibody approach for targeting extracellular and intracellular tau.Brain communications · 2024Article
- Long non-coding RNA ACTA2-AS1 promotes ductular reaction by interacting with the p300/ELK1 complex.Journal of hepatology · 2022Article
- Alu RNA Structural Features Modulate Immune Cell Activation and A-to-I Editing of Alu RNAs Is Diminished in Human Inflammatory Bowel Disease.Frontiers in immunology · 2022Article
- Reduced RNA adenosine-to-inosine editing in hippocampus vasculature associated with Alzheimer's disease.Brain communications · 2022Article
- Cutting Edge: Reduced Adenosine-to-Inosine Editing of Endogenous Alu RNAs in Severe COVID-19 Disease.Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- Reduced A-to-I editing of endogenous Alu RNAs in lung after SARS-CoV-2 infection.Current research in immunology · 2021Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We present a method to synthesize mRNAs from synthetic DNA templates that produce biologically active proteins. To illustrate utility, we constructed five unique synthetic DNA templates, produced mRNAs and demonstrated biologic activity of their translated proteins. Examples include secreted luciferase, enhanced green fluorescence protein, IL-4, and IL-12A and IL-12B to form active IL-12. We propose that this method offers a cost- and time-saving alternative to plasmid-based cloning.
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Registered trials
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