ArticleThe Biochemical journal2020
Source of 12C in Calvin-Benson cycle intermediates and isoprene emitted from plant leaves fed with 13CO2.
Article in The Biochemical journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 54 citations in OpenAlex.
- Carbon Dioxide Release During Photosynthesis: Connecting Gas Exchange Behavior With Biochemistry.Plant, cell & environment · 2026Review
- Time-resolved targeted metabolomics shows an abrupt switch from Calvin-Benson-Bassham cycle to tricarboxylic acid cycle when the light is turned off.Photosynthesis research · 2025Article
- Isoprene deters insect herbivory by priming plant hormone responses.Science advances · 2025Article
- The effects of photosynthetic rate on respiration in light, starch/sucrose partitioning, and other metabolic fluxes within photosynthesis.Scientific reports · 2025Article
- Analysis of the spatial distribution of metabolites in Aloe vera leaves by mass spectrometry imaging and UHPLC-UHRMS.Scientific reports · 2025Article
- Reactive oxygen species-post translational modifications-central carbon metabolism regulatory loop: coordination of redox homeostasis and carbon flux allocation in plants under abiotic stress.Frontiers in plant science · 2025Review
- The end game(s) of photosynthetic carbon metabolism.Plant physiology · 2024Review
- Deoxyxylulose 5-Phosphate Synthase Does Not Play a Major Role in Regulating the Methylerythritol 4-Phosphate Pathway in Poplar.International journal of molecular sciences · 2024Article
- Plastid ancestors lacked a complete Entner-Doudoroff pathway, limiting plants to glycolysis and the pentose phosphate pathway.Nature communications · 2024Article
- Relationship between Cumulative Temperature and Light Intensity and G93 Parameters of Isoprene Emission for the Tropical TreePlants (Basel, Switzerland) · 2024Article
- Using Gas Exchange to Study COMethods in molecular biology (Clifton, N.J.) · 2024Article
- Hydroxymethylbutenyl diphosphate accumulation reveals MEP pathway regulation for high COProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Mechanisms of metabolic adaptation in the duckweed Lemna gibba: an integrated metabolic, transcriptomic and flux analysis.BMC plant biology · 2023Article
- Laisk measurements in the nonsteady state: Tests in plants exposed to warming and variable CO2 concentrations.Plant physiology · 2023Article
- High atmospheric COThe New phytologist · 2022Article
- An Arabidopsis GCMS chemical ionization technique to quantify adaptive responses in central metabolism.Plant physiology · 2022Article
- Compartment-specific energy requirements of photosynthetic carbon metabolism in Camelina sativa leaves.Planta · 2022Article
- Design and fabrication of an improved dynamic flow cuvette forPlant methods · 2022Article
- Reimport of carbon from cytosolic and vacuolar sugar pools into the Calvin-Benson cycle explains photosynthesis labeling anomalies.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Metabolic profiles in C3, C3-C4 intermediate, C4-like, and C4 species in the genus Flaveria.Journal of experimental botany · 2022Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Feeding 14CO2 was crucial to uncovering the path of carbon in photosynthesis. Feeding 13CO2 to photosynthesizing leaves emitting isoprene has been used to develop hypotheses about the sources of carbon for the methylerythritol 4-phosphate pathway, which makes the precursors for terpene synthesis in chloroplasts and bacteria. Both photosynthesis and isoprene studies found that products label very quickly (<10 min) up to 80-90% but the last 10-20% of labeling requires hours indicating a source of 12C during photosynthesis and isoprene emission. Furthermore, studies with isoprene showed that the proportion of slow label could vary significantly. This was interpreted as a variable contribution of carbon from sources other than the Calvin-Benson cycle (CBC) feeding the methylerythritol 4-phosphate pathway. Here, we measured the degree of label in isoprene and photosynthetic metabolites 20 min after beginning to feed 13CO2. Isoprene labeling was the same as labeling of photosynthesis intermediates. High temperature reduced the label in isoprene and photosynthesis intermediates by the same amount indicating no role for alternative carbon sources for isoprene. A model assuming glucose, fructose, and/or sucrose reenters the CBC as ribulose 5-phosphate through a cytosolic shunt involving glucose 6-phosphate dehydrogenase was consistent with the observations.
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