ArticleeLife2020
3D virtual pathohistology of lung tissue from Covid-19 patients based on phase contrast X-ray tomography.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- An Optimized Freeze-Dry Multimodal Workflow for Sequential Micro-CT Imaging, Histology, and Molecular Profiling: A Use Case in Human Liver Fibrosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Non-destructive three-dimensional structural assessment of transbronchial cryobiopsy specimens using phase-contrast X-ray CT: a proof-of-concept study.Translational lung cancer research · 2026Article
- Multi-contrast X-ray microtomography of human lung specimens with an extended field-of-view.Medical physics · 2026Article
- A lung CT vision foundation model facilitating disease diagnosis and medical imaging.Nature communications · 2025Article
- 3D histology of human heart-forming organoids by X-ray phase-contrast tomography.Communications biology · 2025Article
- Imaging technologies in experimental pulmonary fibrosis research: essential tool for enhanced translational relevance.European respiratory review : an official journal of the European Respiratory Society · 2025Review
- Air artifact suppression in phase contrast micro-CT using conditional generative adversarial networks.Journal of synchrotron radiation · 2025Article
- Acute respiratory distress syndrome (ARDS): from mechanistic insights to therapeutic strategies.MedComm · 2025Review
- Cryo-X-Ray Phase Contrast Imaging Enables Combined 3D Structural Quantification and Nucleic Acid Analysis of Myocardial Biopsies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- On the Way to Accounting for Lung Modulation Effects in Particle Therapy of Lung Cancer Patients-A Review.Cancers · 2024Review
- Multiscale X-ray phase-contrast CT uncovers adaptive changes and compensatory mechanisms of circulatory pathways during acute liver injury.Communications biology · 2024Article
- Development of a nanometre scale X-ray speckle-based CT technique through the 3-D histological assessment of an acute respiratory distress syndrome model.Scientific reports · 2024Article
- Emerging multimodality imaging techniques for the pulmonary circulation.The European respiratory journal · 2024Review
- 3D imaging of SARS-CoV-2 infected hamster lungs by X-ray phase contrast tomography enables drug testing.Scientific reports · 2024Article
- Three-dimensional analysis of human pancreatic cancer specimens by phase-contrast based X-ray tomography - the next dimension of diagnosis.Cancer imaging : the official publication of the International Cancer Imaging Society · 2023Article
- Deep Survival Analysis With Clinical Variables for COVID-19.IEEE journal of translational engineering in health and medicine · 2023Article
- Heterotopic mineral deposits in intact rat Achilles tendons are characterized by a unique fiber-like structure.Journal of structural biology: X · 2023Article
- Identifying hub genes and common biological pathways between COVID-19 and benign prostatic hyperplasia by machine learning algorithms.Frontiers in immunology · 2023Article
- A high-resolution 3D atlas of the spectrum of tuberculous and COVID-19 lung lesions.EMBO molecular medicine · 2022Article
- SARS-CoV-2-Infection (COVID-19): Clinical Course, Viral Acute Respiratory Distress Syndrome (ARDS) and Cause(s) of Death.Medical sciences (Basel, Switzerland) · 2022Review
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Authors and funding
11 authors.
Funding
Abstract
We present a three-dimensional (3D) approach for virtual histology and histopathology based on multi-scale phase contrast x-ray tomography, and use this to investigate the parenchymal architecture of unstained lung tissue from patients who succumbed to Covid-19. Based on this first proof-of-concept study, we propose multi-scale phase contrast x-ray tomography as a tool to unravel the pathophysiology of Covid-19, extending conventional histology by a third dimension and allowing for full quantification of tissue remodeling. By combining parallel and cone beam geometry, autopsy samples with a maximum cross section of 8 mm are scanned and reconstructed at a resolution and image quality, which allows for the segmentation of individual cells. Using the zoom capability of the cone beam geometry, regions-of-interest are reconstructed with a minimum voxel size of 167 nm. We exemplify the capability of this approach by 3D visualization of diffuse alveolar damage (DAD) with its prominent hyaline membrane formation, by mapping the 3D distribution and density of lymphocytes infiltrating the tissue, and by providing histograms of characteristic distances from tissue interior to the closest air compartment.
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