ArticleEMBO reports2020
A cell atlas of adult muscle precursors uncovers early events in fibre-type divergence in Drosophila.
Article in EMBO reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 37 citations in OpenAlex.
- Arginine Kinase 1 supports energy homeostasis in Drosophila flight muscle development.PLoS genetics · 2026Article
- Spatiotemporal control of myoblast identity drives muscle diversity in theScience advances · 2026Article
- A single-cell atlas of alternative wing development in two hemipteran species.Nature communications · 2026Article
- Rewiring of two conserved signalling hubs into one axis underlies convergent female-specific wing regression in insects.Nature ecology & evolution · 2026Article
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- Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking.Nature communications · 2026Article
- Single-cell transcriptomics of X-ray irradiatedeLife · 2026Article
- Comparative genomic analysis reveals the genetic basis of the environmental adaptability of Propylea japonica.BMC genomics · 2025Article
- TET exhibits enzymatic-independent and-dependent functions during Drosophila flight muscle development and aging.Skeletal muscle · 2025Article
- Next generation genetic screens in kinetoplastids.Nucleic acids research · 2025Review
- Insufficient wing development possibly contributes to flightlessness of the silkwormProceedings. Biological sciences · 2025Article
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- Amalgam plays a dual role in controlling the number of leg muscle progenitors and regulating their interactions with the developing Drosophila tendon.PLoS biology · 2024Article
- SiFT: uncovering hidden biological processes by probabilistic filtering of single-cell data.Nature communications · 2024Article
- Review
- Review
- Identification of evolutionarily conserved regulators of muscle mitochondrial network organization.Nature communications · 2022Article
- Imaginal Disc Regeneration: Something Old, Something New.Cold Spring Harbor perspectives in biology · 2022Review
- Ets21C sustains a pro-regenerative transcriptional program in blastema cells of Drosophila imaginal discs.Current biology : CB · 2022Article
- Single-Cell Atlas of theInternational journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
In Drosophila, the wing disc-associated muscle precursor cells give rise to the fibrillar indirect flight muscles (IFM) and the tubular direct flight muscles (DFM). To understand early transcriptional events underlying this muscle diversification, we performed single-cell RNA-sequencing experiments and built a cell atlas of myoblasts associated with third instar larval wing disc. Our analysis identified distinct transcriptional signatures for IFM and DFM myoblasts that underlie the molecular basis of their divergence. The atlas further revealed various states of differentiation of myoblasts, thus illustrating previously unappreciated spatial and temporal heterogeneity among them. We identified and validated novel markers for both IFM and DFM myoblasts at various states of differentiation by immunofluorescence and genetic cell-tracing experiments. Finally, we performed a systematic genetic screen using a panel of markers from the reference cell atlas as an entry point and found a novel gene, Amalgam which is functionally important in muscle development. Our work provides a framework for leveraging scRNA-seq for gene discovery and details a strategy that can be applied to other scRNA-seq datasets.
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