ArticleScientific reports2020
Estimation of the number of synapses in the hippocampus and brain-wide by volume electron microscopy and genetic labeling.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 70 citations in OpenAlex.
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- Dendritic morphology and synaptic nonlinearities enhance functional complexity in human cortical neurons.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Region-specific proteomic profiling of brain interstitial fluidLab on a chip · 2026Article
- Review
- Memory engram synapse 3D macromolecular architecture visualized by cryoCLEM-guided cryoET.Structure (London, England : 1993) · 2026Article
- Heterogeneity of astrocyte density, morphology and connexins in the mouse hippocampus.Frontiers in neuroanatomy · 2026Article
- Nondestructive X-ray tomography of brain tissue ultrastructure.Nature methods · 2025Article
- Synaptic MEMOIR: mapping individual synapses of neurons with protein barcodes.bioRxiv : the preprint server for biology · 2025Article
- Cryoprotectants-assisted plunge freezing of thick brain tissue specimens for targeted physiologically relevant cryo-imagingbioRxiv : the preprint server for biology · 2025Article
- Combinatorial protein barcodes enable self-correcting neuron tracing with nanoscale molecular context.bioRxiv : the preprint server for biology · 2025Article
- Central Neurophysiological Alterations in Dystrophic mdx Mice Correlate With Reduced Hippocampal Levels of the Endogenous NMDA Receptor Ligand D-Aspartate.Journal of neurochemistry · 2025Article
- Interactions of Therapeutic Antibodies With Presynaptically-Released Misfolded Proteins in Neurodegenerative Diseases. A Spatial Monte-Carlo Simulation Study.CPT: pharmacometrics & systems pharmacology · 2025Article
- Synaptic Vesicle Glycoprotein 2A Knockout in Parvalbumin and Somatostatin Interneurons Drives Seizures in the Postnatal Mouse Brain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Memory engram synapse 3D molecular architecture visualized by cryoCLEM-guided cryoET.bioRxiv : the preprint server for biology · 2025Article
- Volume electron microscopy analysis of synapses in primary regions of the human cerebral cortex.Cerebral cortex (New York, N.Y. : 1991) · 2024Article
- Tracing nerve fibers with volume electron microscopy to quantitatively analyze brain connectivity.Communications biology · 2024Article
- Bioorthogonal chemical labeling of endogenous neurotransmitter receptors in living mouse brains.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Between neurons and networks: investigating mesoscale brain connectivity in neurological and psychiatric disorders.Frontiers in neuroscience · 2024Review
- The advent of preventive high-resolution structural histopathology by artificial-intelligence-powered cryogenic electron tomography.Frontiers in molecular biosciences · 2024Review
- Information encoded in volumes and areas of dendritic spines is nearly maximal across mammalian brains.Scientific reports · 2023Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
Determining the number of synapses that are present in different brain regions is crucial to understand brain connectivity as a whole. Membrane-associated guanylate kinases (MAGUKs) are a family of scaffolding proteins that are expressed in excitatory glutamatergic synapses. We used genetic labeling of two of these proteins (PSD95 and SAP102), and Spinning Disc confocal Microscopy (SDM), to estimate the number of fluorescent puncta in the CA1 area of the hippocampus. We also used FIB-SEM, a three-dimensional electron microscopy technique, to calculate the actual numbers of synapses in the same area. We then estimated the ratio between the three-dimensional densities obtained with FIB-SEM (synapses/µm
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.