ArticleThe Journal of veterinary medical science2020
Optimal activation methods for maximizing the concentrations of platelet-derived growth factor-BB and transforming growth factor-β1 in equine platelet-rich plasma.
Article in The Journal of veterinary medical science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- Effect of intrauterine administration of platelet-rich plasma two days before timed artificial insemination in dairy cows on endometrial thickness and conception rate.The Journal of veterinary medical science · 2026Article
- Addressing Heterogeneity in Equine PRP Therapies: A Scoping Review of Methods, Evidence, and Commercial Validation.Animals : an open access journal from MDPI · 2025Review
- Arthroscopic PRP gel augmentation at the tendon-bone interface accelerates functional recovery and structural healing in rotator cuff repair: a retrospective cohort study.BMC musculoskeletal disorders · 2025Article
- Protective effect of exosomes derived from platelet-rich plasma during human sperm cryopreservation.Molecular and cellular biochemistry · 2025Article
- Equine bone marrow aspirate and bone marrow aspirate concentrate are enriched with interleukin-1 receptor antagonist protein.American journal of veterinary research · 2025Article
- Article
- Medium-term storage of platelet-derived orthobiologics: a feasible alternative for equine practice.Frontiers in veterinary science · 2025Article
- Use of flow cytometry method to detect contaminations of platelet suspensions.World journal of microbiology & biotechnology · 2024Article
- A comparative study of platelet storage lesion in platelet-rich plasma under cryopreservation.Annals of hematology · 2024Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Platelet-rich plasma (PRP) therapy has been widely applied in various medical fields including humans and horses. This study aimed to establish an optimal activation method to stably and reproducibly maximize the concentrations of platelet-derived growth factor-BB (PDGF-BB) and transforming growth factor-β1 (TGF-β1) contained in equine PRP. Autologous PRP was prepared from 11 Thoroughbreds. For the activation test, PRP was activated by either a single freeze-thaw cycle (Fr) or adding calcium and autologous serum containing thrombin (Ca). PDGF-BB and TGF-β1 concentrations in Fr, Ca, nonactivated (No), and platelet-poor plasma (PPP) samples were determined using ELISA and compared. For repetitive freeze-thaw test, PRP was subjected to single (Fr1), double (Fr2), triple (Fr3), or quadruple (Fr4) freeze-thaw cycles and the concentrations of both growth factors in samples were compared similarly. The PDGF-BB concentration in Ca was significantly higher than that in other preparations. The TGF-β1 concentrations in Fr and Ca were significantly higher than those in PPP and No, with no significant differences between Fr and Ca. The concentrations of both factors were significantly increased in PRP treated with multiple cycles of freeze-thaw compared with that in PRP treated with a single cycle. No significant differences were noted among Fr2, Fr3, and Fr4. Our findings suggest that activation by adding calcium and autologous serum is optimal for instant use of PRP and that double freeze-thawing is an easier and optimal activation method for cryopreserved PRP.
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