ArticlePLoS biology2020
Quantitative cytogenetics reveals molecular stoichiometry and longitudinal organization of meiotic chromosome axes and loops.
Article in PLoS biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 66 citations in OpenAlex.
- Targeted integration of extrachromosomal arrays inScience advances · 2026Article
- Organization of replicated chromosomes by DNA loops and sister chromatid cohesion.Nature reviews. Molecular cell biology · 2026Review
- Early resolution of sister chromatids during C. elegans meiosis.The Journal of cell biology · 2026Article
- Active maintenance of meiosis-specific chromosome structures inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Direct binding of chromosome axis and cohesin complexes underlies meiotic chromosome architecture in fungi and plants.bioRxiv : the preprint server for biology · 2026Article
- Chromosome movements in meiotic prophase: regulatory mechanisms and biological roles.Frontiers in cell and developmental biology · 2026Review
- A DNA-containing phase-separated compartment links the nuclear envelope to chromosome axes to promote homolog pairing inScience advances · 2025Article
- Active maintenance of meiosis-specific chromosome structures inbioRxiv : the preprint server for biology · 2025Article
- Crossover patterning through condensation and coarsening of pro-crossover factors.Nature cell biology · 2025Article
- Cohesin ring gates are specialized for meiotic cell division.Journal of molecular cell biology · 2025Article
- Identification of the Polo-like kinase substrate required for homologous synapsis.The Journal of cell biology · 2025Article
- The synaptonemal complex aligns meiotic chromosomes by wetting.Science advances · 2025Article
- The synaptonemal complex aligns meiotic chromosomes by wetting.bioRxiv : the preprint server for biology · 2024Article
- Canonical and noncanonical roles of Hop1 are crucial for meiotic prophase in the fungus Sordaria macrospora.PLoS biology · 2024Article
- Formation and resolution of meiotic chromosome entanglements and interlocks.Journal of cell science · 2024Review
- The dynamic recruitment of LAB proteins senses meiotic chromosome axis differentiation in C. elegans.The Journal of cell biology · 2024Article
- Automated Quantification of Meiotic Recombination Foci Position and Intensity.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Computational Tools for the Analysis of Meiotic Prophase I Images.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Defining a core configuration for human centromeres during mitosis.Nature communications · 2023Article
- The kleisin subunit controls the function ofeLife · 2023Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
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Abstract
During meiosis, chromosomes adopt a specialized organization involving assembly of a cohesin-based axis along their lengths, with DNA loops emanating from this axis. We applied novel, quantitative, and widely applicable cytogenetic strategies to elucidate the molecular bases of this organization using Caenorhabditis elegans. Analyses of wild-type (WT) chromosomes and de novo circular minichromosomes revealed that meiosis-specific HORMA-domain proteins assemble into cohorts in defined numbers and co-organize the axis together with 2 functionally distinct cohesin complexes (REC-8 and COH-3/4) in defined stoichiometry. We further found that REC-8 cohesins, which load during S phase and mediate sister-chromatid cohesion, usually occur as individual complexes, supporting a model wherein sister cohesion is mediated locally by a single cohesin ring. REC-8 complexes are interspersed in an alternating pattern with cohorts of axis-organizing COH-3/4 complexes (averaging 3 per cohort), which are insufficient to confer cohesion but can bind to individual chromatids, suggesting a mechanism to enable formation of asymmetric sister-chromatid loops. Indeed, immunofluorescence/fluorescence in situ hybridization (immuno-FISH) assays demonstrate frequent asymmetry in genomic content between the loops formed on sister chromatids. We discuss how features of chromosome axis/loop architecture inferred from our data can help to explain enigmatic, yet essential, aspects of the meiotic program.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.