Evidence map›Paper›PMID 32813246›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Methods for the Study of Long Noncoding RNA in Cancer Cell Signaling.

Yi Feng, Junjie Jiang, Zhongyi Hu, Jiao Yuan, Tianli Zhang, Yutian Pan, Mu Xu, Chunsheng Li, Youyou Zhang, Lin Zhang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yi FengCenter for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Junjie JiangCenter for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Zhongyi HuCenter for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Jiao YuanCenter for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Tianli ZhangCenter for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Yutian PanCenter for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Mu XuCenter for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Chunsheng LiCenter for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Youyou ZhangCenter for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Lin ZhangCenter for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Xiaowen HuCenter for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. xiaowenh@pennmedicine.upenn.edu.

Funding

Therapeutic Micro RNA Strategies for Ovarian CancerP50CA083638 · NCI · RESEARCH INST OF FOX CHASE CAN CTR · PI GODWIN, ANDREW K., GOLEMIS, ERICA A. · 1999 to 2013
$33.6M
Targeted Combination Therapy for MelanomaP50CA174523 · NCI · WISTAR INSTITUTE · PI HERLYN, MEENHARD F · 2014 to 2018
$11.1M
Molecular mechanism and preclinical development of BETi and PARPi combination therapyR01CA225929 · NCI · UNIVERSITY OF PENNSYLVANIA · PI ZHANG, LIN · 2019 to 2023
$1.8M
The epigenetic mechanism of long non-coding RNA in cancerR01CA190415 · NCI · UNIVERSITY OF PENNSYLVANIA · PI ZHANG, LIN · 2015 to 2019
$1.8M
The role of microRNA, mir-30d, in the initiation and progression of cancerR01CA142776 · NCI · UNIVERSITY OF PENNSYLVANIA · PI ZHANG, LIN · 2010 to 2014
$1.6M
NCI NIH HHS P50 CA083638NCI NIH HHS P50 CA174523NCI NIH HHS R01 CA142776NCI NIH HHS R01 CA190415NCI NIH HHS R01 CA225929
6 · The paper itself

Abstract

With the advances in sequencing technology and transcriptome analysis, it is estimated that up to 75% of the human genome is transcribed into RNAs. This finding prompted intensive investigations on the biological functions of noncoding RNAs and led to very exciting discoveries of microRNAs as important players in disease pathogenesis and therapeutic applications. Research on long noncoding RNAs (lncRNAs) is in its infancy, yet a broad spectrum of biological regulations has been attributed to lncRNAs. Here, we provide a collection of detailed experimental protocols for lncRNA studies, including lncRNA immunoprecipitation, lncRNA pull-down, lncRNA northern blot analysis, lncRNA in situ hybridization, and lncRNA knockdown. We hope that the information included in this chapter can speed up research on lncRNAs biology and eventually lead to the development of clinical applications with lncRNA as novel prognostic markers and therapeutic targets.

Indexed as

Biomarkers, TumorBlotting, NorthernGene Expression Regulation, NeoplasticGene Knockdown TechniquesGenes, Tumor SuppressorGenome, HumanHumansImmunoprecipitationIn Situ HybridizationMolecular BiologyNeoplasmsOncogenesRNA, Long NoncodingSignal TransductionBiomarkers, TumorRNA, Long NoncodingIn situ hybridizationLong noncoding RNANorthern blotsRNA immunoprecipitationRNA pull-downShort hairpin RNA

Identifiers

PMID32813246
PMCPMC7587466

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.