Evidence map›Paper›PMID 32813064›Full record

ArticleApplied microbiology and biotechnology2020

Hybrid-architectured double-promoter expression systems enhance and upregulate-deregulated gene expressions in Pichia pastoris in methanol-free media.

İrem Demir, Pınar Çalık

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 17 citations in OpenAlex.

  1. Review
  2. Review
  3. Current advances ofFrontiers in microbiology · 2022
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

İrem DemirDepartment of Chemical Engineering, Biochemical Reaction Engineering Laboratory, Middle East Technical University, 06800, Ankara, Turkey.
Pınar ÇalıkDepartment of Chemical Engineering, Biochemical Reaction Engineering Laboratory, Middle East Technical University, 06800, Ankara, Turkey. pcalik@metu.edu.tr.
Middle East Technical University · TR

Funding

Türkiye Bilimsel ve Teknolojik Araştirma Kurumu 116Z215 and 119Z435Türkiye Bilimsel ve Teknolojik Araştirma Kurumu BİDEB Scholarship
6 · The paper itself

Abstract

Double-promoter expression system (DPES) design as de novo metabolic engineering strategy enables fine-tuned and enhanced gene expression. We constructed a collection of monodirectional hybrid-architectured DPESs with engineered promoter variants P

Indexed as

MethanolPichiaGene ExpressionGene Expression Regulation, FungalHumansRecombinant ProteinsSaccharomycetalesMethanolRecombinant ProteinsAlcohol oxidase 1 (AOX1) promoterDeregulated gene expressionDouble-promoter expression system (DPES)Engineered promoterMethanol-free mediaPichia pastoris (Komagataella phaffii)

Identifiers

PMID32813064
OpenAlexW3072412344

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.