Evidence map›Paper›PMID 32812205›Full record

ArticleIn vitro cellular & developmental biology. Animal2020

Cytotoxicity assay using a human pluripotent stem cell-derived cranial neural crest cell model.

Yuichi Mine, Mika Suga, Sumiyo Mimura, Memori Minoda, Takeshi Murayama, Hiroki Nikawa, Miho K Furue

Abstract read
PubMed Publisher
In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 90% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Yuichi MineDepartment of Medical System Engineering, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi Minami-ku, Hiroshima, 734-8553, Japan. mine@hiroshima-u.ac.jp.ORCID http://orcid.org/0000-0002-7057-1955
Mika SugaLaboratory of Stem Cell Cultures, National Institutes of Biomedical Innovation, Health and Nutrition, 7-6-8, Saito-Asagi, Ibaraki, 567-0085, Osaka, Japan.
Sumiyo MimuraDepartment of Oral Biology and Engineering, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi Minami-ku, Hiroshima, 734-8553, Japan.
Memori MinodaDepartment of Medical System Engineering, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi Minami-ku, Hiroshima, 734-8553, Japan.
Takeshi MurayamaDepartment of Medical System Engineering, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi Minami-ku, Hiroshima, 734-8553, Japan.
Hiroki NikawaDepartment of Oral Biology and Engineering, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi Minami-ku, Hiroshima, 734-8553, Japan.
Miho K FurueLaboratory of Stem Cell Cultures, National Institutes of Biomedical Innovation, Health and Nutrition, 7-6-8, Saito-Asagi, Ibaraki, 567-0085, Osaka, Japan.
Hiroshima University · JPNational Institute of Biomedical Innovation, Health and Nutrition · JP

Funding

Japan Agency for Medical Research and Development JP17bk0104011h0005Ministry of Education, Culture, Sports, Science and Technology of Japan 15K11035Ministry of Education, Culture, Sports, Science and Technology of Japan 16H05535Ministry of Education, Culture, Sports, Science and Technology of Japan 17K17181
6 · The paper itself

Abstract

Cleft lip and palate are the most common congenital abnormalities that occur early in pregnancy. The majority of cranial mesenchyme is derived from cranial neural crest cells that differentiate into odontoblasts, cartilage, craniofacial bone, and connective tissue. A subset of these cells differentiates into cranial ganglia. We have previously reported an induction protocol of cranial neural crest cell-like cells from human pluripotent stem cells. This study tested detection of the cytotoxic sensitivities of dental materials, including titanium ions, palladium ions, and hydroxyethyl methacrylate, on the cell viability of induced cranial neural crest cell-like cells (iNC-LCs) derived from Tic human induced pluripotent stem cell (hiPSC) line. Further, the sensitivity was compared with those of human fetal lung fibroblastic cell line MRC-5, which is origin of Tic hiPSC, and osteoblastic cell line MC3T3-E1 which was derived from mouse calvaria. The results suggested that this cell-based assay system using iNC-LCs is a potential method for in vitro screening as an alternative to animal testing to predict toxic effects of dental materials on early craniofacial development.

Indexed as

Models, BiologicalBiological AssayCell DeathCell LineCell SurvivalHumansInduced Pluripotent Stem CellsMethacrylatesNeural CrestPalladiumSkullTitaniumhydroxyethyl methacrylateMethacrylatesPalladiumTitaniumCranial mesenchymeCranial neural crest cellsCytotoxicity assayDefined culture mediumHuman induced pluripotent stem cells

Identifiers

PMID32812205
OpenAlexW3058055418

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.