Evidence map›Paper›PMID 32812133›Full record

ArticleGraefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie2021

Detection of systemic immunosuppressants in autologous serum eye drops (ASED) in patients with severe chronic ocular graft versus host disease.

Volkan Tahmaz, Martin H J Wiesen, Uta Gehlsen, Laura Sauerbier, Michael E Stern, Udo Holtick, Birgit Gathof, Christof Scheid, Carsten Müller, Philipp Steven

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.4field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 13 citations in OpenAlex.

  1. Serum Eye Drops: Implementation, Upscaling, and Patient-Centered Care - Insights from a Blood Bank in Western Austria.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2026
    Review
  2. Article
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  5. [Chinese expert consensus on ocular chronic graft-versus-host disease (2023)].Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi · 2023
    Article
  6. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Volkan TahmazDivision for Dry-eye disease and ocular GvHD, Department of Ophthalmology, University of Cologne Medical Faculty and University Hospital of Cologne, Cologne, Germany.
Martin H J WiesenCenter of Pharmacology, Department of Therapeutic Drug Monitoring, University Hospital of Cologne, Cologne, Germany.
Uta GehlsenDivision for Dry-eye disease and ocular GvHD, Department of Ophthalmology, University of Cologne Medical Faculty and University Hospital of Cologne, Cologne, Germany.
Laura SauerbierDivision for Dry-eye disease and ocular GvHD, Department of Ophthalmology, University of Cologne Medical Faculty and University Hospital of Cologne, Cologne, Germany.
Michael E SternDivision for Dry-eye disease and ocular GvHD, Department of Ophthalmology, University of Cologne Medical Faculty and University Hospital of Cologne, Cologne, Germany.
Udo HoltickDepartment of Internal Medicine I, University Hospital Cologne, Cologne, Germany.
Birgit GathofInstitute of Transfusion Medicine, University of Cologne, Cologne, Germany.
Christof ScheidDepartment of Internal Medicine I, University Hospital Cologne, Cologne, Germany.
Carsten MüllerCenter of Pharmacology, Department of Therapeutic Drug Monitoring, University Hospital of Cologne, Cologne, Germany.
Philipp StevenDivision for Dry-eye disease and ocular GvHD, Department of Ophthalmology, University of Cologne Medical Faculty and University Hospital of Cologne, Cologne, Germany. philipp.steven@uk-koeln.de.
University of Cologne · DEUniversity Hospital Cologne · DECologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeChronic graft versus host disease is a major consequence after allogeneic stem cell transplantation (allo-SCT) and has great impact on patients' morbidity and mortality. Besides the skin, liver, and intestines, the eyes are most commonly affected, manifesting as severe ocular surface disease. Treatment protocols include topical steroids, cyclosporine, tacrolimus, and ASED. Since these patients often receive systemic immunosuppressant therapy from their oncologists, a topical re-administration of these drugs via ASED with potentially beneficial or harmful effects is possible. The purpose of the study was to determine whether and to which extent systemic immunosuppressants are detectable in ASED.

methodsA total of 34 samples of ASED from 16 patients with hemato-oncological malignancies after allo-SCT were collected during the manufacturing process and screened for levels of cyclosporine, mycophenolic acid, everolimus, and tacrolimus via liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The study followed the tenets of the Declaration of Helsinki and informed consent was obtained from the subjects after explanation of the nature and possible consequences of the study.

resultsCyclosporine was found in 18 ASED samples in concentrations ranging from 6.5-105.0 ng/ml (32.0 ± 22.8 ng/ml, mean ± SD). The concentration range of mycophenolic acid in 19 samples was 0.04-25.0 mg/l (4.0 ± 5.4 mg/l, mean ± SD). Everolimus and tacrolimus concentrations were well below the respective limits of quantification (< 0.6 and < 0.5 ng/ml) of the established LC-MS/MS method in all samples.

conclusionsOur study suggests that orally administered cyclosporine and mycophenolic acid for the treatment of systemic GvHD, but not everolimus and tacrolimus, are distinctly detectable in ASED in relevant concentrations. It is highly likely that these agents affect topical therapy of ocular GvHD. However, the extent of this effect needs to be evaluated in further studies.

Indexed as

Graft vs Host DiseaseImmunosuppressive AgentsChromatography, LiquidCyclosporineHumansOphthalmic SolutionsTacrolimusTandem Mass SpectrometryCyclosporineImmunosuppressive AgentsOphthalmic SolutionsTacrolimusAutologous serum eye dropsGraft-versus-host diseaseImmunosuppression; mass spectrometry

Identifiers

PMID32812133
PMCPMC7790777
OpenAlexW3073988136

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.