ArticleScientific reports2020
Reference gene selection for qRT-PCR analysis of season- and tissue-specific gene expression profiles in the honey bee Apis mellifera.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 49 citations in OpenAlex.
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- Reference gene selection for RT-qPCR normalization in the drone testis of honey bee (Apis cerana) during meiosis stages.PloS one · 2026Article
- The serotonin receptor AmFrontiers in physiology · 2026Article
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- Selection and validation of RT-qPCR reference genes for multi-tissue gene expression normalization in two honeybee subspecies across post-emergence developmental stages.BMC genomics · 2025Article
- Differences in the suitability of published honey bee (Apis mellifera) reference genes between the African subspecies Apis mellifera scutellata and European derived Apis mellifera.Molecular ecology · 2025Article
- Honey bee (Apis mellifera) queen quality: host-microbial transcriptomes exploring the influence of age and hindgut symbiont Commensalibacter melissae.Animal microbiome · 2025Article
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- Novel fungicide and neonicotinoid insecticide impair flight behavior in pollen foraging honey bees, Apis mellifera.Scientific reports · 2024Article
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- Metabolism gene expression in worker honey bees after exposure to 50Hz electric field - semi-field analysis.Frontiers in zoology · 2024Article
- Honey bee colony behavior and ontogeny are adversely affected when exposed to a pesticide-contaminated environment.Journal of insect science (Online) · 2024Article
- A longitudinal study of queen health in honey bees reveals tissue specific response to seasonal changes and pathogen pressure.Scientific reports · 2024Article
- Evaluation of reference genes for quantitative expression analysis inFrontiers in physiology · 2024Article
- Effects of ingested essential oils and propolis extracts on honey bee (Hymenoptera: Apidae) health and gut microbiota.Journal of insect science (Online) · 2023Article
- Cellular heterogeneity of the developing worker honey bee (Apis mellifera) pupa: a single cell transcriptomics analysis.G3 (Bethesda, Md.) · 2023Article
- Resilin Distribution and Abundance inInsects · 2023Article
- Nanoparticle-Shielded dsRNA Delivery for Enhancing RNAi Efficiency in Cotton Spotted BollwormInternational journal of molecular sciences · 2023Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Honey bees are both important pollinators and model insects due to their highly developed sociality and colony management. To better understand the molecular mechanisms underlying honey bee colony management, it is important to investigate the expression of genes putatively involved in colony physiology. Although quantitative real-time PCR (qRT-PCR) can be used to quantify the relative expression of target genes, internal reference genes (which are stably expressed across different conditions) must first be identified to ensure accurate normalisation of target genes. To identify reliable reference genes in honey bee (Apis mellifera) colonies, therefore, we evaluated seven candidate genes (ACT, EIF, EF1, RPN2, RPS5, RPS18 and GAPDH) in samples collected from three honey bee tissue types (head, thorax and abdomen) across all four seasons using three analysis programmes (NormFinder, BestKeeper and geNorm). Subsequently, we validated various normalisation methods using each of the seven reference genes and a combination of multiple genes by calculating the expression of catalase (CAT). Although the genes ranked as the most stable gene were slightly different on conditions and analysis methods, our results suggest that RPS5, RPS18 and GAPDH represent optimal honey bee reference genes for target gene normalisation in qRT-PCR analysis of various honey bee tissue samples collected across seasons.
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