ArticleMicrobial cell factories2020
Successful biosynthesis of natural antioxidant ergothioneine in Saccharomyces cerevisiae required only two genes from Grifola frondosa.
Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 58 citations in OpenAlex.
- Ergothioneine: Biosynthesis, Molecular Mechanisms, Physiological Function, and Role in Disease.MedComm · 2026Review
- Efficient Synthesis of Ergothioneine in Escherichia coli Through Identification of Bacterial-Type Egt1 and Self-Assemble of Pathway Enzymes.Biotechnology and bioengineering · 2026Article
- EngineeringMicroorganisms · 2026Article
- From Production to Application: Postbiotics in Meat, Meat Products, Other Food Matrices, and Bioactive Packaging.Foods (Basel, Switzerland) · 2026Review
- Enhancement of ergothioneine production in Corynebacterium glutamicum by increasing osmotic pressure.Applied microbiology and biotechnology · 2025Article
- CRISPR/Cas9-based iterative multi-copy integration for improved metabolite yields inSynthetic and systems biotechnology · 2025Article
- Biosynthesis of ergothioneine: current state, achievements, and perspectives.Applied microbiology and biotechnology · 2025Review
- Ergothioneine biosynthesis: The present state and future prospect.Synthetic and systems biotechnology · 2025Review
- The Catalytic Mechanism of Key Enzymes Involved in the Synthesis of Ergothioneine inMolecules (Basel, Switzerland) · 2024Article
- AdvancingBioengineering (Basel, Switzerland) · 2024Review
- Influence of Storage Temperature on Levels of Bioactive Compounds in Shiitake Mushrooms (Mycobiology · 2023Article
- A SingleInternational journal of molecular sciences · 2022Article
- Enhancement of ergothioneine production by discovering and regulating its metabolic pathway in Cordyceps militaris.Microbial cell factories · 2022Article
- Toward more efficient ergothioneine production using the fungal ergothioneine biosynthetic pathway.Microbial cell factories · 2022Article
- Ergothioneine, Ovothiol A, and Selenoneine-Histidine-Derived, Biologically Significant, Trace Global Alkaloids.Molecules (Basel, Switzerland) · 2022Review
- Ergothioneine, recent developments.Redox biology · 2021Article
- Antioxidant Properties and Industrial Uses of Edible Polyporales.Journal of fungi (Basel, Switzerland) · 2021Review
- Microbial Cell Factory of Baccatin III Preparation inFrontiers in microbiology · 2021Article
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6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundErgothioneine (EGT) has a unique antioxidant ability and diverse beneficial effects on human health. But the content of EGT is very low in its natural producing organisms such as Mycobacterium smegmatis and mushrooms. Therefore, it is necessary to highly efficient heterologous production of EGT in food-grade yeasts such as Saccharomyces cerevisiae.
resultsTwo EGT biosynthetic genes were cloned from the mushroom Grifola frondosa and successfully heterologously expressed in Saccharomyces cerevisiae EC1118 strain in this study. By optimization of the fermentation conditions of the engineered strain S. cerevisiae EC1118, the 11.80 mg/L of EGT production was obtained. With daily addition of 1% glycerol to the culture medium in the fermentation process, the EGT production of the engineered strain S. cerevisiae EC1118 can reach up to 20.61 mg/L.
conclusionA successful EGT de novo biosynthetic system of S. cerevisiae containing only two genes from mushroom Grifola frondosa was developed in this study. This system provides promising prospects for the large scales production of EGT for human health.
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