ArticleGigaScience2020
A haplotype-resolved, de novo genome assembly for the wood tiger moth (Arctia plantaginis) through trio binning.
Article in GigaScience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Independent Major-Effect Loci and Limited Trait Associations Characterize Warning Colour Variation Across Sexes and Life Stages in the Wood Tiger Moth (Arctia plantaginis).Molecular ecology · 2026Article
- Genetic and phenotypic variation in wood tiger moths from the Caucasus: insights into male warning color variation.Insect science · 2026Article
- Telomere-to-telomere genome assembly of the wood tiger moth Arctia plantaginis.Scientific data · 2026Article
- High-quality, haplotype-resolved reference genomes of the Dutch warmblood horse and Friesian horse using trio binning.BMC genomics · 2025Article
- Haplotyped genome mapping and functional characterization of a blueberry anthocyanin acetyltransferase (AAT) controlling the accumulation of acylated anthocyanins.Journal of experimental botany · 2025Article
- PISAD: reference-free intraspecies sample anomalies detection tool based on k-mer counting.GigaScience · 2025Article
- The Hypolimnas misippus Genome Supports a Common Origin of the W Chromosome in Lepidoptera.Genome biology and evolution · 2024Article
- The ancestral karyotype of the Heliantheae Alliance, herbicide resistance, and human allergens: Insights from the genomes of common and giant ragweed.The plant genome · 2024Article
- Hybrid-hybrid correction of errors in long reads with HERO.Genome biology · 2023Article
- Article
- Representing true plant genomes: haplotype-resolved hybrid pepper genome with trio-binning.Frontiers in plant science · 2023Article
- A chromosome-level, haplotype-phased Vanilla planifolia genome highlights the challenge of partial endoreplication for accurate whole-genome assembly.Plant communications · 2022Article
- RNA Virosphere in a Marine Zooplankton Community in the Subtropical Western North Pacific.Microbes and environments · 2022Article
- Chromosome-Level Haplotype Assembly forFrontiers in genetics · 2022Article
- Antibiotics accelerate growth at the expense of immunity.Proceedings. Biological sciences · 2021Article
- Correction to: A haplotype-resolved, de novo genome assembly for the wood tiger moth (Arctia plantaginis) through trio binning.GigaScience · 2021Article
- A haplotype-resolved, de novo genome assembly for the wood tiger moth (Arctia plantaginis) through trio binning.GigaScience · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
11 authors.
Funding
Abstract
backgroundDiploid genome assembly is typically impeded by heterozygosity because it introduces errors when haplotypes are collapsed into a consensus sequence. Trio binning offers an innovative solution that exploits heterozygosity for assembly. Short, parental reads are used to assign parental origin to long reads from their F1 offspring before assembly, enabling complete haplotype resolution. Trio binning could therefore provide an effective strategy for assembling highly heterozygous genomes, which are traditionally problematic, such as insect genomes. This includes the wood tiger moth (Arctia plantaginis), which is an evolutionary study system for warning colour polymorphism.
findingsWe produced a high-quality, haplotype-resolved assembly for Arctia plantaginis through trio binning. We sequenced a same-species family (F1 heterozygosity ∼1.9%) and used parental Illumina reads to bin 99.98% of offspring Pacific Biosciences reads by parental origin, before assembling each haplotype separately and scaffolding with 10X linked reads. Both assemblies are contiguous (mean scaffold N50: 8.2 Mb) and complete (mean BUSCO completeness: 97.3%), with annotations and 31 chromosomes identified through karyotyping. We used the assembly to analyse genome-wide population structure and relationships between 40 wild resequenced individuals from 5 populations across Europe, revealing the Georgian population as the most genetically differentiated with the lowest genetic diversity.
conclusionsWe present the first invertebrate genome to be assembled via trio binning. This assembly is one of the highest quality genomes available for Lepidoptera, supporting trio binning as a potent strategy for assembling heterozygous genomes. Using our assembly, we provide genomic insights into the geographic population structure of A. plantaginis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.