Evidence map›Paper›PMID 32808361›Full record

ArticleCell proliferation2020

Exosomal miR-130a-3p regulates osteogenic differentiation of Human Adipose-Derived stem cells through mediating SIRT7/Wnt/β-catenin axis.

Shude Yang, Shu Guo, Shuang Tong, Xu Sun

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell proliferation, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed, 1 pooled it
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Shude YangDepartment of Plastic Surgery, The First Hospital of China Medical University, Shenyang, China.ORCID https://orcid.org/0000-0002-7926-7394
Shu GuoDepartment of Plastic Surgery, The First Hospital of China Medical University, Shenyang, China.
Shuang TongDepartment of Plastic Surgery, The First Hospital of China Medical University, Shenyang, China.
Xu SunDepartment of Plastic Surgery, The First Hospital of China Medical University, Shenyang, China.
First Hospital of China Medical University · CN

Funding

China Postdoctoral Science Foundation 2018M641741National Natural Science Foundation of China 51872332Natural Science Foundation of Liaoning Province 20170541040
6 · The paper itself

Abstract

objectivesIt is of profound significance for clinical bone regeneration to clarify the specific molecular mechanism from which we found that osteogenic differentiation of adipose-derived stem cells (ADSCs) will be probably promoted by exosomes. MATERIALS AND

methodsBy means of lentiviral transfection, miR-130a-3p overexpression and knockdown ADSCs were constructed. Alizarin Red S was used to detect the calcium deposits, and qPCR was used to detect osteogenesis-related genes, to verify the effect of miR-130a-3p on the osteogenic differentiation of ADSCs. CCK-8 was used to detect the effect of miR-130a-3p on the proliferation of ADSCs. The target binding between miR-130a-3p and SIRT7 was verified by dual-luciferase reporter gene assay. Furthermore, the role of Wnt signalling pathway in the regulation of ADSCs osteogenesis and differentiation by miR-130a-3p was further verified by detecting osteogenic-related genes and proteins and alkaline phosphatase activity.

results(a) Overexpression of miR-130a-3p can enhance the osteogenic differentiation of ADSCs while reducing protein and mRNA levels of SIRT7, a target of miR-130a-3p. (b) Our study further found that overexpression of miR-130a-3p leads to down-regulation of SIRT7 expression with up-regulation of Wnt signalling pathway-associated protein. (c) Overexpression of miR-130a-3p inhibited proliferation of ADSCs, while knockdown promoted it.

conclusionsThe obtained findings indicate that exosomal miR-130a-3p can promote osteogenic differentiation of ADSCs partly by mediating SIRT7/Wnt/β-catenin axis, which will hence promote the application of exosomal microRNA in the field of bone regeneration.

Indexed as

OsteogenesisWnt Signaling Pathwaybeta CateninCell DifferentiationCells, CulturedExosomesGene Expression RegulationHumansMesenchymal Stem CellsMicroRNAsSignal TransductionSirtuinsWnt Proteinsbeta CateninMicroRNAsMIRN130 microRNA, humanSIRT7 protein, humanSirtuinsWnt ProteinsAdipose-derived stem cellsbone regenerationexosomesmiR-130a-3pWnt/β-catenin

Identifiers

PMID32808361
PMCPMC7574877
OpenAlexW3081377501

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.