Evidence map›Paper›PMID 32807907›Full record

ArticleNature protocols2020

Engineering DNA nanostructures for siRNA delivery in plants.

Huan Zhang, Honglu Zhang, Gozde S Demirer, Eduardo González-Grandío, Chunhai Fan, Markita P Landry

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
2.6field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 75 citations in OpenAlex.

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  15. DNA-based nanostructures for RNA delivery.Medical review (2021) · 2024
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  19. Application of Nanotechnology in Plant Genetic Engineering.International journal of molecular sciences · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Huan Zhang *Department of Chemical and Biomolecular Engineering, University of California Berkeley, Berkeley, CA, USA.ORCID http://orcid.org/0000-0002-1096-4255
Honglu Zhang *California Institute for Quantitative Biosciences (QB3), University of California Berkeley, Berkeley, CA, USA.
Gozde S DemirerDepartment of Chemical and Biomolecular Engineering, University of California Berkeley, Berkeley, CA, USA.ORCID http://orcid.org/0000-0002-3007-1489
Eduardo González-GrandíoDepartment of Chemical and Biomolecular Engineering, University of California Berkeley, Berkeley, CA, USA.ORCID http://orcid.org/0000-0002-2059-4542
Chunhai FanSchool of Chemistry and Chemical Engineering, and Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Markita P LandryDepartment of Chemical and Biomolecular Engineering, University of California Berkeley, Berkeley, CA, USA. landry@berkeley.edu.ORCID http://orcid.org/0000-0002-5832-8522
QB3 · USUniversity of California, Berkeley · USShanghai Jiao Tong University · CN

Funding

Single-Molecule Imaging of Biological Trauma:Cytokine-Based Intracellular CommunicationR35GM128922 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI LANDRY, MARKITA · 2019 to 2023
$2.0M
NIGMS NIH HHS R35 GM128922
6 · The paper itself

Abstract

Targeted downregulation of select endogenous plant genes is known to confer disease or pest resistance in crops and is routinely accomplished via transgenic modification of plants for constitutive gene silencing. An attractive alternative to the use of transgenics or pesticides in agriculture is the use of a 'green' alternative known as RNAi, which involves the delivery of siRNAs that downregulate endogenous genes to confer resistance. However, siRNA is a molecule that is highly susceptible to enzymatic degradation and is difficult to deliver across the lignin-rich and multi-layered plant cell wall that poses the dominant physical barrier to biomolecule delivery in plants. We have demonstrated that DNA nanostructures can be utilized as a cargo carrier for direct siRNA delivery and gene silencing in mature plants. The size, shape, compactness and stiffness of the DNA nanostructure affect both internalization into plant cells and subsequent gene silencing efficiency. Herein, we provide a detailed protocol that can be readily adopted with standard biology benchtop equipment to generate geometrically optimized DNA nanostructures for transgene-free and force-independent siRNA delivery and gene silencing in mature plants. We further discuss how such DNA nanostructures can be rationally designed to efficiently enter plant cells and deliver cargoes to mature plants, and provide guidance for DNA nanostructure characterization, storage and use. The protocol described herein can be completed in 4 d.

Indexed as

EngineeringDNADrug CarriersNanostructuresNicotianaRNA, Small InterferingDNADrug CarriersRNA, Small Interfering

Identifiers

PMID32807907
PMCPMC10493160
OpenAlexW3049169753

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.