ArticleNature communications2020
Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 37 citations in OpenAlex.
- Cohesin reconstitution and homologous recombination repair of DNA double-strand breaks in late mitosis.eLife · 2025Article
- PBK/TOPK mediates Ikaros, Aiolos and CTCF displacement from mitotic chromosomes and alters chromatin accessibility at selected C2H2-zinc finger protein binding sites.Nature communications · 2025Article
- Hbo1 and Msl complexes preserve differential compaction and H3K27me3 marking of active and inactive X chromosomes during mitosis.Nature cell biology · 2025Article
- Molecular mechanisms altering cell identity in cancer.Oncogene · 2025Review
- L-2-hydroxyglutarate regulates centromere and heterochromatin conformation in the male germline.PLoS genetics · 2025Article
- Integrated promoter-capture Hi-C and Hi-C analysis reveals fine-tuned regulation of the 3D chromatin architecture in colorectal cancer.Frontiers in genetics · 2025Article
- The molecular basis of cell memory in mammals: The epigenetic cycle.Science advances · 2024Review
- Cell and developmental biology: grand challenges.Frontiers in cell and developmental biology · 2024Article
- Release of Histone H3K4-reading transcription factors from chromosomes in mitosis is independent of adjacent H3 phosphorylation.Nature communications · 2023Article
- Identification of mammalian transcription factors that bind to inaccessible chromatin.Nucleic acids research · 2023Article
- GATA2 mitotic bookmarking is required for definitive haematopoiesis.Nature communications · 2023Article
- Heat shock transcription factors demonstrate a distinct mode of interaction with mitotic chromosomes.Nucleic acids research · 2023Article
- Genome folding dynamics during the M-to-G1-phase transition.Current opinion in genetics & development · 2023Review
- Mitotic bookmarking by SWI/SNF subunits.Nature · 2023Article
- Decoding protein methylation function with thermal stability analysis.Nature communications · 2023Article
- Loss of H3K9 trimethylation alters chromosome compaction and transcription factor retention during mitosis.Nature structural & molecular biology · 2023Article
- Regulation of the mitotic chromosome folding machines.The Biochemical journal · 2022Article
- Article
- Maintaining Transcriptional Specificity Through Mitosis.Annual review of genomics and human genetics · 2022Review
- Function and regulation of transcription factors during mitosis-to-G1 transition.Open biology · 2022Review
Corrections and comments
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Authors and funding
20 authors at 5 institutions in 1 country.
Funding
Abstract
Epigenetic information is transmitted from mother to daughter cells through mitosis. Here, to identify factors that might play a role in conveying epigenetic memory through cell division, we report on the isolation of unfixed, native chromosomes from metaphase-arrested cells using flow cytometry and perform LC-MS/MS to identify chromosome-bound proteins. A quantitative proteomic comparison between metaphase-arrested cell lysates and chromosome-sorted samples reveals a cohort of proteins that were significantly enriched on mitotic ESC chromosomes. These include pluripotency-associated transcription factors, repressive chromatin-modifiers such as PRC2 and DNA methyl-transferases, and proteins governing chromosome architecture. Deletion of PRC2, Dnmt1/3a/3b or Mecp2 in ESCs leads to an increase in the size of individual mitotic chromosomes, consistent with de-condensation. Similar results were obtained by the experimental cleavage of cohesin. Thus, we identify chromosome-bound factors in pluripotent stem cells during mitosis and reveal that PRC2, DNA methylation and Mecp2 are required to maintain chromosome compaction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.