Evidence map›Paper›PMID 32806745›Full record

ArticleMolecules (Basel, Switzerland)2020

Triplexed CEA-NSE-PSA Immunoassay Using Time-Gated Terbium-to-Quantum Dot FRET.

Shashi Bhuckory, K David Wegner, Xue Qiu, Yu-Tang Wu, Travis L Jennings, Anne Incamps, Niko Hildebrandt

Open access · goldAbstract read
In one paragraph

Article in Molecules (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 4 countries.

Shashi BhuckoryCEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, 91198 Gif-sur-Yvette, France.
K David WegnerFederal Institute for Materials Research and Testing (BAM), Division Biophotonics, Richard-Willstaetter-Strasse 11, 12489 Berlin, Germany.
Xue QiuCEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, 91198 Gif-sur-Yvette, France.
Yu-Tang WuCEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, 91198 Gif-sur-Yvette, France.
Travis L JenningsThermo Fisher Scientific, 5781 Van Allen Way, Carlsbad, CA 92008, USA.
Anne IncampsThermo Fisher Scientific Cezanne SAS, Clinical Diagnostic Division, 30000 Nimes, France.
Niko HildebrandtCEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, 91198 Gif-sur-Yvette, France.
CEA Paris-Saclay · FRInstitut de Biologie Intégrative de la Cellule · FRFederal Institute For Materials Research and Testing · DEThermo Fisher Scientific (United States) · US

Funding

Agence Nationale de la Recherche ANR project "Neutrinos" and Labex "SynOrg"Horizon 2020 Framework Programme Marie Sklodowska-Curie grant agreement No. 846764Institut Universitaire de France Membership Niko HildebrandtRégion Normandie Chaire d'Excellence COBRA-FRET
6 · The paper itself

Abstract

Time-gated Förster resonance energy transfer (TG-FRET) between Tb complexes and luminescent semiconductor quantum dots (QDs) provides highly advantageous photophysical properties for multiplexed biosensing. Multiplexed Tb-to-QD FRET immunoassays possess a large potential for in vitro diagnostics, but their performance is often insufficient for their application under clinical conditions. Here, we developed a homogeneous TG-FRET immunoassay for the quantification of carcinoembryonic antigen (CEA), neuron-specific enolase (NSE), and prostate-specific antigen (PSA) from a single serum sample by multiplexed Tb-to-QD FRET. Tb-IgG antibody donor conjugates were combined with compact QD-F(ab')

Indexed as

Fluorescence Resonance Energy TransferCarcinoembryonic AntigenGPI-Linked ProteinsHumansImmunoassayImmunoglobulin GKallikreinsProstate-Specific AntigenQuantum DotsTerbiumCarcinoembryonic AntigenCEACAM5 protein, humanGPI-Linked ProteinsImmunoglobulin GKallikreinsKLK3 protein, humanProstate-Specific AntigenTerbiumbiosensingCEAfluorescencelanthanidesmultiplexingnanoparticlesNSEPSA

Identifiers

PMID32806745
PMCPMC7464126
OpenAlexW3048821256

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.