Evidence map›Paper›PMID 32806670›Full record

ArticleInternational journal of environmental research and public health2020

Comprehensive Fungal Community Analysis of House Dust Using Next-Generation Sequencing.

Kazuki Izawa, Atsutaka Kubosaki, Naoki Kobayashi, Yutaka Akiyama, Akiko Yamazaki, Kazuhiro Hashimoto, Rumi Konuma, Yoichi Kamata, Yukiko Hara-Kudo, Kenichi Hasegawa and 2 more

Abstract read
In one paragraph

Article in International journal of environmental research and public health, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Review
  3. Assembly and network ofChinese herbal medicines · 2025
    Article
  4. Resistome in the indoor dust samples from workplaces and households: a pilot study.Frontiers in cellular and infection microbiology · 2024
    Article
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Kazuki IzawaDepartment of Computer Science, School of Computing, Tokyo Institute of Technology, Meguro-ku, Tokyo 152-8550, Japan.
Atsutaka KubosakiDivision of Microbiology, National Institute of Health Sciences, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan.
Naoki KobayashiDepartment of Food and Life Science, School of Life and Environmental Science, Azabu University, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.
Yutaka AkiyamaDepartment of Computer Science, School of Computing, Tokyo Institute of Technology, Meguro-ku, Tokyo 152-8550, Japan.ORCID 0000-0003-2863-8703
Akiko YamazakiDepartment of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, Japan.
Kazuhiro HashimotoLaboratory of Integrated Pest Management, FCG Research Institute, Inc., Koto-ku, Tokyo 135-0064, Japan.
Rumi KonumaTokyo Metropolitan Industrial Technology Research Institute, Koto-ku, Tokyo 135-0064, Japan.
Yoichi KamataDepartment of Food Design, Faculty of Nutritional Science, Koshien University, Takarazuka, Hyogo 665-0006, Japan.
Yukiko Hara-KudoDivision of Microbiology, National Institute of Health Sciences, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan.
Kenichi HasegawaDepartment of Architecture and Environment Systems, Faculty of Systems Science and Technology, Akita Prefectural University, Yurihonjo, Akita 015-0055, Japan.
Toshiharu IkagaDepartment of System Design Engineering, Faculty of Science and Technology, Keio University, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan.ORCID 0000-0002-3451-5614
Maiko WatanabeDivision of Microbiology, National Institute of Health Sciences, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fungal community analyses in homes have been attracting attention because fungi are now generally considered to be allergens. Currently, these analyses are generally conducted using the culture method, although fungal communities in households often contain species that are difficult to culture. In contrast, next-generation sequencing (NGS) represents a comprehensive, labor- and time-saving approach that can facilitate species identification. However, the reliability of the NGS method has not been compared to that of the culture method. In this study, in an attempt to demonstrate the reliability of this application, we used the NGS method to target the internal transcribed spacer 1 (ITS1) in the fungal genome, conducted fungal community analyses for 18 house-dust samples and analyzed fungal community structures. The NGS method positively correlated with the culture method regarding the relative abundance of

Indexed as

AllergensDustFungiMycobiomeHigh-Throughput Nucleotide SequencingReproducibility of ResultsAllergensDustfungal community analysishouse dustITS regionnext-generation sequencing

Identifiers

PMID32806670
PMCPMC7460106

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.