ArticleiScience2020
Functional Characterization of Three-Dimensional Cortical Cultures for In Vitro Modeling of Brain Networks.
Article in iScience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Human blood-brain barrier tissue model to characterize lipid nanoparticle delivery and transport mechanisms.Biomaterials · 2026Article
- AAV-delivered TurboRFP enables streamlined tracing and analysis of corticospinal tract sprouting.Cell reports methods · 2026Article
- Three-dimensional microelectrode arrays forBiomedical engineering letters · 2026Review
- Molecular abnormalities scale across three models of cerebral injury.Frontiers in molecular neuroscience · 2026Article
- Multiscale Organization of Neural Networks in a 3D Bioprinted Matrix.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Electrophysiological features of cortical 3D networks are deeply modulated by scaffold properties.APL bioengineering · 2024Article
- Investigating the interplay between segregation and integration in developing cortical assemblies.Frontiers in cellular neuroscience · 2024Article
- N-Methyl-D-Aspartate (NMDA) Receptor Antagonists and their Pharmacological Implication: A Medicinal Chemistry-oriented Perspective Outline.Current medicinal chemistry · 2024Review
- Rheological Characterization of Three-Dimensional Neuronal Cultures Embedded in PEGylated Fibrin Hydrogels.Gels (Basel, Switzerland) · 2023Article
- Long-term calcium imaging reveals functional development in hiPSC-derived cultures comparable to human but not rat primary cultures.Stem cell reports · 2023Article
- Building blocks of microphysiological system to model physiology and pathophysiology of human heart.Frontiers in physiology · 2023Review
- Functional bioengineered models of the central nervous system.Nature reviews bioengineering · 2023Review
- A 3D-induced pluripotent stem cell-derived human neural culture model to study certain molecular and biochemical aspects of Alzheimer's disease.In vitro models · 2022Article
- High throughput 3D gel-based neural organotypic model for cellular assays using fluorescence biosensors.Communications biology · 2022Article
- Recent progresses in novelFrontiers in bioengineering and biotechnology · 2022Review
- Operationalizing the Use of Biofabricated Tissue Models as Preclinical Screening Platforms for Drug Discovery and Development.SLAS discovery : advancing life sciences R & D · 2021Article
- Learning and synaptic plasticity in 3D bioengineered neural tissues.Neuroscience letters · 2021Article
- Integrated functional neuronal network analysis of 3D silk-collagen scaffold-based mouse cortical culture.STAR protocols · 2021Article
- Electrophysiology Read-Out Tools for Brain-on-Chip Biotechnology.Micromachines · 2021Review
- Neuronal Graphs: A Graph Theory Primer for Microscopic, Functional Networks of Neurons Recorded by Calcium Imaging.Frontiers in neural circuits · 2021Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Three-dimensional (3D) in vitro cultures recapitulate key features of the brain including morphology, cell-cell and cell-extracellular matrix interactions, gradients of factors, and mechanical properties. However, there remains a need for experimental and computational tools to investigate network functions in these 3D models. To address this need, we present an experimental system based on 3D scaffold-based cortical neuron cultures in which we expressed the genetically encoded calcium indicator GCaMP6f to record neuronal activity at the millimeter-scale. Functional neural network descriptors were computed with graph-theory-based network analysis methods, showing the formation of functional networks at 3 weeks of culture. Changes to the functional network properties upon perturbations to glutamatergic neurotransmission or GABAergic neurotransmission were quantitatively characterized. The results illustrate the applicability of our 3D experimental system for the study of brain network development, function, and disruption in a biomimetic microenvironment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.