ReviewiScience2020
Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease.
Review in iScience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
59 citing papers in PubMed.
- Three-Dimensional Architecture of Ectopic Epithelium and Vasculature in Ovarian Endometriosis Revealed by Tissue-Clearing Imaging.Cell proliferation · 2026Article
- A uniform tissue-clearing framework and mesoSPIM-ultra enable cm-scale single-neuron tracing.bioRxiv : the preprint server for biology · 2026Article
- SynClear: A one-step synchronous clearing and labeling strategy for multiscale 3D brain mapping.Materials today. Bio · 2026Article
- Advanced tissue clearing and three-dimensional imaging approaches to visualize neural innervation in the rat knee joints.Npj imaging · 2026Article
- Protocol for 3D-guided sectioning and deep cell phenotyping via light sheet imaging and 2D spatial multiplexing.STAR protocols · 2026Article
- Multidimensional lung imaging: integrated preclinical platforms enabling the identification of translational biomarkers for pulmonary research.Respiratory research · 2026Review
- Fetal Mouse Heart Clearing, Imaging, and 3D Reconstruction.Methods in molecular biology (Clifton, N.J.) · 2026Article
- 3D vascular mapping reveals multi-organ injury following severe acute pancreatitis.Theranostics · 2026Article
- Immunohistochemical Localization and Anatomical Distribution of Enteric Glia Cells in Enteric Nervous System.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Clinical applications of artificial intelligence in the histopathology of lymphoma: diagnosis, treatment and prognosis.Discover oncology · 2025Review
- In-depth 3D exploration of autosomal dominant polycystic kidney disease through light sheet fluorescence microscopy.Scientific reports · 2025Article
- Rapid sonication-assisted whole tissue clearing and immunostaining.Scientific reports · 2025Article
- ADAPT-3D:accelerated deep adaptable processing of tissue for 3-dimensional fluorescence tissue imaging for research and clinical settings.Scientific reports · 2025Article
- Imaging 3D cell cultures with optical microscopy.Nature methods · 2025Review
- Review
- From morphology to single-cell molecules: high-resolution 3D histology in biomedicine.Molecular cancer · 2025Review
- In vivo optical tissue clearing using light-absorbing dyes.Lab animal · 2024Article
- High-Speed Clearing and High-Resolution Staining for Analysis of Various Markers for Neurons and Vessels.Tissue engineering and regenerative medicine · 2024Article
- Advanced Immunolabeling Method for Optical Volumetric Imaging Reveals Dystrophic Neurites of Dopaminergic Neurons in Alzheimer's Disease Mouse Brain.Molecular neurobiology · 2024Article
- BiliQML: a supervised machine-learning model to quantify biliary forms from digitized whole slide liver histopathological images.American journal of physiology. Gastrointestinal and liver physiology · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three-dimensional (3D) optical imaging techniques can expand our knowledge about physiological and pathological processes that cannot be fully understood with 2D approaches. Standard diagnostic tests frequently are not sufficient to unequivocally determine the presence of a pathological condition. Whole-organ optical imaging requires tissue transparency, which can be achieved by using tissue clearing procedures enabling deeper image acquisition and therefore making possible the analysis of large-scale biological tissue samples. Here, we review currently available clearing agents, methods, and their application in imaging of physiological or pathological conditions in different animal and human organs. We also compare different optical tissue clearing methods discussing their advantages and disadvantages and review the use of different 3D imaging techniques for the visualization and image acquisition of cleared tissues. The use of optical tissue clearing resources for large-scale biological tissues 3D imaging paves the way for future applications in translational and clinical research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.